Based on our complete carbohydrate metabolism analysis solutions, CD BioGlyco provides sorbitol analysis services by high-performance liquid chromatography (HPLC) and high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD).
Sorbitol, also known as D-glucitol, is a six-carbon sugar alcohol with sweet taste. Most sorbitol is made from potato starch, but it also occurs naturally in the plant kingdom and many fruits, such as apples, cherries, plums, and pears. Sorbitol has been around for over 60 years as a commercial product because of its unique physical and chemical properties. Today, sorbitol has been used in food, medical, and other industries. In the food industry, sorbitol is used in desserts, ice cream, and chewing gum as a sweetener, humectant, and thickener. In the medical industry, sorbitol is the raw material for antiseptic drugs. In addition, sorbitol can also be used in laxatives.
Fig.1 Sorbitol structure. (Wikipedia)
Sorbitol stores and transports sugars in plants as one of the end products of photosynthesis. It is synthesized from glucose under the action of sorbitol-6-phosphate dehydrogenase (S6PDH) and sorbitol-6-pyrophosphatase (S6PP). In the human body, externally obtained sorbitol is slowly absorbed into the body from the gastrointestinal tract, and then absorbed and metabolized in the liver as fructose by a pathway located entirely in the cytoplasmic compartment. Studies have shown that excess sorbitol in the body may be converted into glucose, which then accumulates in the kidneys causing kidney, nerve tissue, and retinal damage.
Fig.2 Sorbitol metabolism. (Sheet, 2014)
Sorbitol is a naturally occurring polyol that is widely used in the food and pharmaceutical industries. CD BioGlyco has been focusing on carbohydrate research for many years and has developed complete Carbohydrate Metabolism Analysis solutions. We provide sorbitol analysis services for fruits, vegetables, food, and plants. Our methods include:
HPLC is the most common chromatographic technique for the determination of sugar content. We can determine sorbitol content in various samples by HPLC with refractive index detection (HPLC-RID), HPLC with evaporative light scattering detector (HPLC-ELSD), and HPLC-mass spectrometry (HPLC-MS).
Compared to HPLC, HPAEC-PAD has higher sensitivity and is suitable for the determination of sorbitol content in low-level samples.
CD BioGlyco is a leader in carbohydrate research, providing accurate, reliable, and reproducible sorbitol analysis services for our global clients. If you are interested in our services, please feel free to contact us, we are looking forward to being your research assistant in the field of carbohydrates.