Natamycin Analysis Service

Your Partner for Precise Natamycin Analysis

Natamycin, also known as natamycin, is a naturally occurring antifungal agent with antibiotic properties produced by Actinomyces spp. This compound exhibits broad-spectrum activity against a wide range of fungi and yeasts. Although natamycin has significant antifungal activity, in practical application, an in-depth study of its antifungal mechanism of action and symptomatic use is essential. This is because different fungi have different sensitivities to natamycin and may be resistant to it. CD BioGlyco offers comprehensive a diverse array of Pharmaceutical Analysis services, which encompass the Quinidine Gluconate Analysis Service, Sisomicin Sulfate Analysis Service, and natamycin analysis service. Our natamycin analysis service lies:

  • We employ a rapid, highly sensitive, and robust liquid chromatography-mass spectrometry (LC-MS/MS) technique for the analysis of natamycin. This method combines LC and MS techniques to accurately detect and quantify the chemical constituents in natamycin samples. The LC-MS/MS technique allows us to obtain high-resolution, high-sensitivity data and the ability to detect multiple target substances simultaneously.
  • Meanwhile, we conduct a comprehensive analysis of the natamycin content using various spectroscopic techniques including infrared spectroscopy(IR), ultraviolet-visible absorption spectrum (UV-Vis), and nuclear magnetic resonance(NMR) Additionally, chromatographic analysis methods such as high-performance liquid chromatography (HPLC) and gas chromatography (GC) are employed to further investigate the composition of a compound.
  • In addition to the aforementioned services, we also utilize a range of other advanced biochemical analytical techniques for the detection and analysis of natamycin. One such technique is enzyme-linked immunosorbent assay (ELISA), which allows us to directly measure the presence and concentration of natamycin in various samples. ELISA is highly sensitive and specific, providing accurate results that are crucial for quality control purposes. Furthermore, we employ immunofluorescence as another powerful tool for both direct and indirect detection of natamycin. This involves labeling specific antibodies with fluorescent dyes, allowing us to visualize and identify the presence of natamycin within samples under a fluorescence microscope. Immunofluorescence not only provides qualitative information about the distribution of natamycin but can also be quantified using image analysis software.

Fig.1 Technologies in natamycin analysis. (CD BioGlyco) Fig.1 Technologies in natamycin analysis. (CD BioGlyco)

Publication Data

Technology: Immunoassay and ELISA

Journal: Biosensors

IF: 5.743

Published: 2022

Results: This article focuses on an immunoassay method for the detection of antifungal drug natamycin traces in food. The method employs specific antibodies that selectively bind to natamycin and quantifies its concentration in the sample by measuring the signal generated from the immunoreaction, thereby enabling rapid screening. The article verifies that cyclodextrins (CDs) as food additives do not have a significant effect on the determination of natamycin. The presence of CDs in the natamycin assay does not alter the natamycin measurement. In addition, since organic solvents destroyed the CD-natamycin complexes, the CD-natamycin complexes were destroyed during the extraction of natamycin from food samples and did not affect the detection of natamycin. The article also developed an ELISA variant for the extraction of natamycin from different food matrices. The extraction process was optimized, solvents were used, and different food matrices were tested. The recovery of the extraction process was 72-106% with a variability of less than 12%. The ELISA variant had the highest sensitivity (IC50 = 0.12 ng/mL) with a detection limit of 0.02 ng/mL for the natamycin assay. Thus, this ELISA variant can be used to extract natamycin from different food matrices with high sensitivity and detection limits. The recovery and variability of the extraction process were also within acceptable limits.

Fig.2 Structures of NAT-based immunogens and coating antigens for indirect competitive ELISA. (Burkin, et al., 2022) Fig.2 Structures of natamycin-based immunogens and coating antigens for indirect competitive ELISA. (Burkin, et al., 2022)


  • Our combination of analytical techniques allows us to comprehensively analyze natamycin samples from a variety of perspectives, providing additional information and data support.
  • Our professional team provides in-depth interpretation of the analysis results and provides detailed reports and conclusions to help clients understand the analyzed data and make decisions.
  • By integrating effective analytical methods and streamlined workflows, we offer economical analytical solutions that effectively reduce our clients' expenditure of time.

CD BioGlyco offers one-stop and customized natamycin analysis services, aiming to make a valuable contribution to your antifungal drug research involving natamycin. If you are interested in our services, we warmly invite you to contact us for further comprehensive information.


  1. Burkin, M.A.; et al. Immunoassay for natamycin trace screening: bread, wine and other edibles analysis. Biosensors. 2022, 12(7): 493.
This service is for Research Use Only, not intended for any clinical use.

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