Application of Miniature Pig Kidney N-Glycan-Immobilized Beads for High-Throughput (HT) Glycan Screening

Miniature pig kidney N-glycan-immobilized beads are prepared based on polysaccharide material. These beads have a smaller particle size, which provides a higher surface area and better separation. Its surface is attached with N-glycans from the micro pig kidney. Also, its surface is immobilized with miniature pig kidney N-glycans, which have the ability to specifically interact with the glycan chain structure. By incubating the glycan mixture to be screened with the beads, the glycan molecules specifically bind to miniature pig kidney N-glycans immobilized on the surface of the microbeads. High-throughput screening (HTS) of glycans using this technology allows for the simultaneous processing of a large number of samples and improves screening efficiency. During the screening process, different wash buffers can be used to remove non-specific bound glycan molecules, improving the specificity of the screening results.

Fig.1 General procedure for the screening of glycan-binding proteins. (Kim, et al., 2008)

HT Glycan Screening Using Miniature Pig Kidney N-Glycan-Immobilized Beads at CD BioGlyco

At CD BioGlyco, we combine miniature pig kidney N-glycan-immobilized beads with other analytical techniques to rapidly screen large numbers of samples. This makes it possible to screen multiple glycan samples at the same time, speeding up the screening process and increasing efficiency. Our specific services are listed below:

• We combined the beads with Mass Spectrometry analysis to achieve the HTS of glycans. The target glycans are enriched using the affinity interaction between the immobilized glycosylated structures on the bead surface and the target glycans. Enriched glycans are then dissociated from the bead surface by elution. Finally, the eluted glycans are structurally identified and quantitatively analyzed by mass spectrometry.
• We combined beads with High-Performance Liquid Chromatography (HPLC) to enrich the target glycans through the affinity of the glycosylated structures immobilized on the surface of the beads for the target glycans. HPLC is then used for separation and quantification. The separation is achieved by selecting a suitable column and mobile phase according to the chemical properties and molecular size of the glycan samples. Finally, the peak areas of various glycans are quantified by a detector.

Fig.2 Flow chart of HT analysis of glycans. (CD BioGlyco)

Applications

• Drug development and efficacy assessment
• Regulating protein stability in bioengineering
• Glycan structure identification and glycomics studies

Advantages of Us

• Highly specificity
• Various state-of-the-art technology platforms
• Customized experimental procedures on demand

CD BioGlyco offers HT glycan screening service using miniature pig kidney N-glycan-immobilized Beads. We utilize advanced technology and adjust our protocols according to your specific needs. We hope that our reliable service and accurate experimental results will help you accelerate your relevant research. If you have any questions, please feel free to contact us. We look forward to working with you to advance the development of glycosyl research.