Gentamicin Sulfate Analysis Service

Gentamicin Sulfate Analysis Service

Dive Deep into Gentamicin Sulfate (GS) Analysis Excellence

GS is an organic substance with the chemical formula C60H125O22N15·H2SO4. It has the appearance of a white or off-white crystalline powder and dissolves easily in water. In biology and medicine, GS has a favorable inhibitory effect on most bacteria. At CD BioGlyco, we provide professional GS analysis services with our rigorous and extensive knowledge of Pharmaceutical and Biological.

  • Specific rotation
    We accurately weigh a sample of GS and dissolve and dilute it with water. Next, the specific rotation of GS is tested using a spinometer. GS should have a specific rotation of +107° to +121°.
  • Solubility
    GS is soluble in water and insoluble in ethanol, acetone, or ether.
  • Identification
    • Thin layer chromatography (TLC): We will dissolve the sample to be tested and GS standard in water and make it into separate solutions. Next, both solutions are unfolded on the same thin layer plate using the same unfolding agent. Finally, we compare the spots of the sample and the standard in iodine vapor. If the number, location, and color of the spots match, the sample is proven to be GS.
    • Infrared spectroscopy (IR): Infrared light absorption spectra of the control sample and the standard of GS are compared, if the spectra are the same, it proves that the sample is GS.
  • Acidity
    The pH of an aqueous solution of GS is determined directly using an acidimeter. The pH of GS should be 4.0-6.0.
  • Sulfate
    GS is weighed precisely and dissolved in water. We titrate it using barium chloride solution as titrant and phthalide violet solution as indicator solution. Finally, the amount of sulfate in GS is calculated based on the color change and the amount of titrant used.
  • Quantitative analysis
    We use high-performance liquid chromatography (HPLC) to determine the content of GS. The content of GS is calculated using the area of the peak of GS in the chromatogram obtained.
  • Moisture
    We determine the moisture content of GS using a combination of a moisture meter and an electrochemical method.
  • Bacterial endotoxins
    We offer gel and photometric methods for the determination of endotoxin in GS. These two methods mainly utilize the agglutination phenomenon and turbidity change during the reaction of horseshoe crab reagent with endotoxin to determine the amount of endotoxin.

Fig.1 GS analysis services. (CD BioGlyco)Fig.1 GS analysis services. (CD BioGlyco)

GS is also commonly used in medical applications in several forms, and CD BioGlyco offers its analysis to clients. Notably, most of their analysis aspects are the same as those of GS.

  • GS tablets: White to light yellow tablets or sugar-coated tablets, white to light yellow after removing the coating
  • GS injection: A sterile aqueous solution of GS as a colorless to slightly yellow or slightly yellowish-green clear liquid with a pH between 3.5 and 6.0
  • GS sustained-release tablets: White or off-white tablets
  • GS granules: Soluble granules
  • GS eye drops: A clear colorless slightly yellow liquid with a pH between 5.0 and 7.0

Fig.2 Several common types of GS. (CD BioGlyco)Fig.2 Several common types of GS. (CD BioGlyco)

Publication Data

Technology: Cross-linking method, Nuclear magnetic resonance (NMR)

Journal: International Journal of Biological Macromolecules

IF: 8.2

Published: 2019

Results: The authors crosslinked two alginate gels of different compositions with Ca2+ and exposed them to GS at different times and concentrations. The authors then found that GS was effective in improving the physicochemical properties of the alginate network by determining the mechanical properties of the formed complexes, NMR spectra, and pH, among others, and that the extent of these changes was related to the structural composition of the polysaccharides.

Fig.3 Alteration of alginate properties during antibiotic administration. (Heriot, et al., 2019)Fig.3 Alteration of alginate properties during antibiotic administration. (Heriot, et al., 2019)


  • The analysis of GS lays the foundation for its bioactivity research. Our analysis helps to further advance the development and modification of the functionality of GS.
  • The analysis of GS can be used to research sepsis, respiratory infections, and other diseases caused by Staphylococcus aureus, Escherichia coli, and other sensitive bacteria.
  • The analysis of GS helps to explore the relationship between its structure and biological function and helps researchers delve deeper into the scope of applications of GS in the field of medicine.


  • We have a team of experienced and professional pharmaceutical analysts with efficient and high-quality analysis speed.
  • We provide analysis services in various fields of glycan-related Pharmaceuticals and offer custom analysis solutions according to the needs of our clients.
  • We have first-class pharmaceutical analysis techniques to assess the properties of samples more accurately.

CD BioGlyco provides our clients with trusted support and assistance in pharmaceutical analysis. Each of our analysis protocols undergoes rigorous quality testing to ensure that we efficiently help our clients solve the challenges they encounter in their research. If you have any further questions, please feel free to contact us.


  1. Heriot, M.; et al. Interaction of gentamicin sulfate with alginate and consequences on the physico-chemical properties of alginate-containing biofilms. International Journal of Biological Macromolecules. 2019, 121: 390-397.
This service is for Research Use Only, not intended for any clinical use.

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