O-GlcNAcase (OGA) Inhibitor Development Service at CD BioGlyco

CD BioGlyco is committed to providing high-quality Glycosylation Inhibitor Development services. O-GlcNAc glycosylation modification is a type of post-translational modification prevalent in prokaryotes and eukaryotes. Throughout the process, OGA is responsible for hydrolyzing GlcNAc off the protein. It is a hexosaminidase present in the cytoplasm and nucleus. O-GlcNAc glycosylation modification associates and participates in the regulation of a variety of cellular processes, including transcription and translation, nutrient and stress perception, neuronal function, cell cycle, etc. The development of O-GlcNAc-based inhibitors is necessary. We provide a one-shop OGA inhibitor development services.

Fig.1 OGA inhibitor development service. (CD BioGlyco)

Most of the OGA inhibitors developed today are competitive inhibitors, being analogs of GlcNAc or reaction intermediates. They bind the active center of the enzyme competitively with the substrate. Based on the types of OGA inhibitors reported, we screen, design, and synthesize compounds that inhibit OGA.

• O-(2-Acetamido-2-deoxy-d-glucopyranosylidene)amino-N-phenylcarbamate (PUGNAc) derivatives

The N-phenyl carbamate portion of PUGNAc captures more binding energy, allowing it to better embed itself in the enzyme pocket and thus inhibit OGA enzyme activity. We design and synthesize PUGNAc derivatives and test their inhibitory effects.

• 1,2-Dideoxy-2'-methyl-α-d-glucopyranoso[2,1-d]-Δ2'-thiazoline (NAG-thiazoline) derivatives

NAG-thiazoline is a transition state analog of OGA hydrolysis and better inhibits human OGA. We design and synthesize NAG-thiazoline derivatives and test their inhibitory effect.

• Other types of inhibitors: e.g. Iminocyclitiols-like inhibitors, Nagstain derivatives, etc.

We synthesize compound libraries using the chick-chemistry approach. This is followed by a high-throughput screening for enzyme activity, which leads to the identification of compounds that effectively inhibit OGA enzyme activity. Finally, we conduct a series of cytological experiments on the compounds. In addition, we use computer-aided design and synthesis of potentially active analogs by studying the crystal structure of the OGA homologous enzyme. The focus of our development is on reducing Ki and improving inhibition and specificity.

Publication

Applications of OGA Inhibitor Development

• The use of highly selective OGA inhibitors allows the study of the mechanisms by which O-GlcNAc regulates mitochondria, among others.
• Important links between O-GlcNAc glycosylation modifications and disease have been identified. The developed OGA inhibitors are used to study specific molecular mechanisms.

Advantages of Us

• We provide customized services for OGA inhibitor development projects as per the client's requirements and use tailor-made experimental procedures to meet specific needs.
• Our development of OGA inhibitors for glycobiological studies avoids some of the problems associated with the use of mutant studies and makes the experiments easier.

CD BioGlyco has been supporting research in the field of glycobiology through a series of high-quality products and related services. Our experienced scientists and technicians will do their best to provide you with the best inhibitor development services. Please feel free to contact us to inquire about the development direction and details of OGA inhibitor development. We look forward to hearing from you. We will be happy to serve you.