Separation of Plant Polysaccharides
Plant polysaccharides play an increasingly important role in pathology and pharmacology, CD BioGlyco provides advanced and alternative polysaccharide separation methods to meet customers' needs, and we have confidence to be your essential research assistant in the field of glycobiology.
Plant polysaccharides refer to complex and large carbohydrates synthesized by the condensation and dehydration of multiple monosaccharide molecules in plants. Its structure usually contains linear and branched chains, with a molecular weight distribution in the range of tens of thousands to millions. More studies have been conducted on plant polysaccharides in recent years, and polysaccharides have been extracted from hundreds of plants.
These plant polysaccharides have essential and unique biological activities, including signal recognition, cell connection, immune system regulation, and blood coagulation. At present, numerous studies have reported that they also have a wide range of pharmacological activities, such as anti-tumor, anti-virus, cardiovascular protection, etc. Polysaccharides with multiple functional chemical groups (hydroxyl, amino and carboxylic acid group) can be further modified for the use in biomedical field, such as vaccine adjuvants, drug carriers, tissue engineering, etc. However, due to the complexity of its functions and structures, the understanding of plant polysaccharides is still very limited, and the important prerequisite for the study of plant polysaccharides is high-quality purification and separation.
The segregation of botanical polysaccharides constitutes a multistage protocol demanding strategic orchestration calibrated to precise source matrices and target analytes. We harness sophisticated methodologies to guarantee exemplary yield and molecular homogeneity.
Our sequence commences with judicious selection of optimal liberation techniques, deploying both classical and contemporary approaches:
An economically viable tradition employing heated aqueous media to solubilize polar carbohydrate ensembles.
Site-specific biocatalysts hydrolyze lignocellulosic matrices, enhancing liberation efficacy under mild regimes that conserve polysaccharide integrity.
This contemporary approach employs microwave radiation to induce rapid thermal excitation and internal pressurization within cellular architectures, provoking structural failure for accelerated constituent release.
High-frequency sonic waves generate inertial cavitation and mechanical agitation, compromising membranous barriers to expedite solute diffusion.
Post-extraction crude isolates contain heterogeneous admixtures—proteinaceous matter, chromophores, and extraneous contaminants. Our refinement cascade executes contaminant excision via:
Foundational fractionation leveraging differential solubility in high-titer aliphatic alcohols.
An exceptionally efficient, gentle protein excision preserving covalent frameworks and bioactivity.
Diverse modalities—including ion-exchange resins—separate carbohydrates through charge-based fractionation and hydrodynamic volumetrics, achieving exceptional molecular homogeneity.
Pressure-driven membranous sieving segregates macromolecular solutes from low-molecular-weight impurities.
The main principle of plant polysaccharide extraction is to break the cell wall from outside to inside under mild conditions, thereby avoiding polysaccharide denaturation. Polysaccharides extracted from natural products contain many impurities, such as inorganic salts, proteins, lignin, etc. Therefore, certain measures need to be taken to separate crude polysaccharides and the mixture of different polysaccharides needs to be purified into a single polysaccharide component with the same degree of polymerization and spatial conformation.
We use the following methods for plant polysaccharides separation.
Our comprehensive plant polysaccharides separation service follows a streamlined, multi-stage workflow designed for maximum efficiency and quality.
We begin with an in-depth consultation to understand your project goals and requirements. Our team analyzes the source material to determine its composition and the most effective extraction and purification strategy.
Based on our analysis, we select and optimize a single or a combination of extraction methods to maximize the yield of your target polysaccharide. This stage is crucial for capturing the polysaccharide while preserving its biological activity.
The crude extract is subjected to an initial purification phase to remove major impurities, such as lipids and proteins, using methods like Sevage and decolorization.
Using a combination of advanced techniques, including column chromatography and ultrafiltration, we meticulously isolate and fractionate the target polysaccharide, achieving the specified purity level.
The final product is rigorously tested to confirm its structure, molecular weight, and purity. Our characterization methods include determining sugar content and other structural properties.
DOI.: 10.1080/19476337.2018.1518343
Journal: CYTA-Journal of Food
Published: 2018
Results: In this study, the authors developed a comprehensive guideline detailing standardized methods for extracting, isolating, purifying, and structurally characterizing polysaccharides from natural resources. The protocol covers diverse extraction techniques (hot water, acid, alkaline) tailored to polysaccharide solubility, followed by purification strategies like membrane filtration, alcohol precipitation, and column chromatography (e.g., ion exchange, gel permeation). Structural analysis methods include molecular weight determination via HPSEC, monosaccharide composition by HPAEC-PAD, glycosidic linkage analysis through methylation and GC-MS, and advanced NMR techniques for sequence elucidation. The guideline emphasizes optimizing purity while preserving structural integrity, enabling reproducible study of polysaccharides' bioactivities and functional properties for food science and pharmaceutical applications.
Our separation service of plant polysaccharides enables the isolation of high-purity carbohydrate fractions from complex botanical sources. To further support detailed structural and functional evaluation, we offer a specialized Polysaccharide Analysis Service that includes glucan profiling, galactan characterization, and xylan composition studies for comprehensive biomolecular investigation.
CD BioGlyco provides a variety of methods to meet the research needs of customers, and customize different experimental programs according to the characteristics of different samples. We use professionalism and efficiency to complete customer's orders and save time and funds for customers while obtaining credible results.
Customers can contact our employees directly and we will respond promptly. If you are interested in our services, please contact us for more detailed information.
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