Based on our complete carbohydrate metabolism analysis solutions, CD BioGlyco provides glucose analysis services in fruits, vegetables, nectar, cheese, and more.
Glucose is mainly a monosaccharide produced by plants and most algae under photosynthesis. Glucose is the most abundant carbohydrate in the world and the most widely used aldohexose in living organisms. It was first isolated from raisins in 1747 by the German chemist Andreas Marggraf, mainly in the form of D-glucose. Natural glucose exists mainly in the free state, while glucose in animals and plants mainly exists in the form of polymers. In animals, glucose mainly comes from the decomposition of glycogen, which is the most important energy source in animals. In plants, glucose is mainly stored in the form of starch and amylopectin. Currently, glucose can be used to produce fructose and glucose-containing products, etc.
Fig.1 D-Glucose structure. (Wikipedia)
Carbohydrates, lipids, and proteins in the organism are broken down into glucose to provide energy for the body. The metabolism of glucose involves glycolysis, the tricarboxylic acid cycle, the pentose phosphate pathway, gluconeogenesis, and glycogen synthesis and breakdown. Glycolysis, the process of converting glucose into pyruvate and releasing energy, is the first step in the process of glucose metabolism in all biological cells. The tricarboxylic acid cycle is the process by which pyruvic acid produces carbon dioxide and water under aerobic conditions and releases energy. The pentose phosphate pathway is another way of oxidative breakdown of glucose in the cytoplasm. Gluconeogenesis can convert non-sugar substances such as lactose, pyruvate and glycerol into glucose. Glycogenolysis produces glucose, which can also be synthesized into glycogen.
Fig.2 Glucose metabolic pathways in the heart. (Tran, 2019)
Sugars have many roles in all aspects of plant and animal life, serving as structural materials, participating in metabolism, producing energy, synthesizing macromolecules, and more. Therefore, the development of sugar analysis methods is very necessary. At CD BioGlyco, we have developed complete Carbohydrate Metabolism Analysis solutions, providing glucose analysis services in fruits, vegetables, nectar, cheese, and more. Our methods include:
HPLC is the most common chromatographic technique for the determination of monosaccharide content in various samples. Not only can we determine the glucose content in nectar by HPLC with refractive index detection (HPLC-RID), but we can also determine the glucose content in fruit by HPLC with an evaporative light scattering detector (HPLC-ELSD).
Compared with the HPLC, HPAEC-PAD has higher sensitivity. It is suitable for the determination of glucose content in dairy products, cheese, and other samples.
CD BioGlyco provides accurate, reliable, and reproducible glucose analysis services for our global clients. If you are interested in our services, please feel free to contact us.