At CD BioGlyco, we leverage over specialized expertise in glycobiology to offer a comprehensive glycomic characterization service. Our service is meticulously designed to elucidate the precise structures and profiles of glycans present in various biological samples, providing researchers and pharmaceutical developers with critical insights into glycan function, biomarker identification, and drug development.

Overview

Carbohydrates are involved in most biological processes in cells, and any alterations in glycan structure or composition may be a cause or consequential attribute of biochemical imbalance. Therefore in current glycobiology research, it is increasingly significant to understand the structural details of biologically important polysaccharides. During the past years, sophisticated mass spectrometry techniques and instrumentation have been developed rapidly in the biomedical field. While these developments have been driven primarily by the demand in the fields of protein science and proteomic research, contemporary glycobiology has also benefited from these developments.

Characterization of the glycome is a key part of understanding the biological basis of health and disease. A number of technologies and methods have been developed to help decipher glycosylation and elucidate glycomic changes. At present, novel mass analyzers and tandem mass spectrometry techniques are now being used to determine the sequence, linkage, chain branching, and other aspects of oligosaccharide structures with unprecedented sensitivity and mass resolution.

Key Technologies

We employ high-performance liquid chromatography (HPLC) for efficient separation of glycan species, often coupled with fluorescence detection for enhanced sensitivity. Mass spectrometry (MS), including techniques like Matrix-assisted laser desorption/ionization time-of-flight MS (MALDI-TOF MS), electrospray ionization tandem-MS (ESI-MS/MS), and LC-MS/MS, is central to our approach, enabling precise mass determination, structural elucidation, and quantitative profiling of glycans.

Comprehensive Glycomic Characterization Service: Unlocking Biological Insights

CD BioGlyco has made significant progress in the development of powerful structural tools to probe even the most complex structural properties of various intracellular and intercellular signaling and regulation. We offer a wide range of services to our clients in the field of glycomics, including glycan profiling, glycan sequencing, and glycan structure analysis.

Our glycomic characterization service follows a meticulously designed, multi-stage workflow to ensure accurate, reproducible, and comprehensive analysis of glycans from diverse biological samples. Our service process is as follows:

• Sample Preparation

Specific protein denaturation, lipid removal, and nucleic acid extraction protocols are employed to ensure optimal glycan accessibility and purity.

• Glycan Release and Derivatization

N-glycans are enzymatically released using PNGase F, and O-glycans are released using reductive β-elimination or non-reducing chemical methods. Derivatization or permethylation with fluorescent markers is then performed to enhance detection sensitivity.

• Glycan Separation and Detection

High-performance liquid chromatography (HPLC) is used to separate complex glycan mixtures, while liquid chromatography-mass spectrometry (LC-MS) is used to isolate and detect individual glycan species.

• Structural Elucidation via MS

Matrix-assisted laser desorption ionization time-of-flight MS (MALDI-TOF-MS) is used to precisely measure glycan masses, and tandem MS (MS/MS) analyzes monosaccharide composition, linkage types, and branching patterns.

• Data Analysis and Report

We utilize specialized software and databases to interpret and validate glycan profiles and structures and provide a comprehensive report detailing the experimental procedures, raw data, and processed results.

Workflow

Publication Data

Applications

• Glycomic characterization services are used in immunology and vaccine development to elucidate the role of glycans in immune recognition and host-pathogen interactions.
• Glycomic characterization services can be used to investigate altered glycosylation patterns in cancer cells to assess tumor progression, metastasis, and drug resistance.
• Glycomic characterization services can be used to explore the role of glycans in neural development, synaptic plasticity, and neurodegenerative diseases.
• Glycomic characterization services can be used to analyze glycans in food for nutritional assessment, allergenicity studies, and the development of functional foods.

Advantages

• Our team of highly skilled scientists brings extensive experience in handling diverse sample types and tackling challenging glycomic questions, ensuring reliable and insightful results.
• We have high-resolution MS and advanced chromatographic systems. This enables us to achieve superior sensitivity, accuracy, and comprehensive coverage in glycan detection and structural elucidation.
• Our service encompasses a broad spectrum of glycan analysis, from detailed N- and O-glycan profiling to precise glycan sequencing and complex structural analysis, including isoform and spatial organization studies. This holistic approach ensures that all aspects of your glycomic characterization needs are met.

Frequently Asked Questions

CD BioGlyco is your trusted partner in glycobiology, offering a comprehensive glycomic characterization service that provides unparalleled insights into the intricate world of glycans. From precise profiling and sequencing to detailed structural elucidation, our advanced technological platforms and expert scientific team are dedicated to supporting your research and development endeavors across diverse applications. Please feel free to contact us to discuss your specific project needs.

Associated Services

To further support your research and development goals, CD BioGlyco offers a suite of complementary services and specialized options related to glycomics:

• Glycoprotein Analysis: Beyond glycan characterization, we offer comprehensive analysis of intact glycoproteins, including glycosylation site mapping and occupancy determination.
• Glycan Array Services: Utilize our high-throughput glycan array platforms for rapid screening of glycan-binding interactions, essential for drug discovery and immunology studies.
• Carbohydrate Vaccine Development: Leverage our expertise in designing and characterizing glycoconjugate vaccines for infectious disease prevention.