High-Versatility LiGA-based Cell Surface Lectin-binding Profiling

CD BioGlyco has extensive experience in Liquid Glycan Display Array (LiGA). The multivalent glycan display is generated on phage by glycanization modification. These detailed services for probing cell surface receptors using LiGA include preparation, modification, assembly of LiGA library, and high-throughput screening of glycan surface receptors. Siglec belongs to the Ig superfamily (type I lectins), which is mainly expressed on leukocytes except for tumor cells and participates in immune responses related to tumors and microbial infections by recognizing glycans containing sialic acid. Our researchers analyze the linkage specificity of lectin ligands by using cell lines with different glycosylation levels on surfaces.

Typical Binding Services Between Cells and LiGA Libraries

Fig.1 Flow chart of a typical binding assay between cells and LiGA library. (CD BioGlyco)

• Profiling service

We have optimized the key parameters of the LiGA-based cell surface lectin-binding profiling service. We perform polymerase chain reaction (PCR) amplification using primers and isogenic cell lines. Moreover, we provide deep-sequencing and differential enrichment analysis services.

• In vivo service

Our researchers have analyzed in detail the biodistribution of LiGA in organs in mice and its association with two subpopulations of B cells. We offer detection services of interactions between lectin ligands and receptors displayed on organ and cell surfaces in living animals. After enrichment, B-cell populations are isolated from the organs. Plaque forming units (PFU) assay service helps clients to analyze the composition of libraries from different organs with B-cell types.

Why Choose LiGA-based Cell Surface Lectin-binding Profiling?

• LiGA-based cell surface lectin-binding profiling effectively detects the specificity-binding in their natural environment.
• LiGA-based cell surface lectin-binding profiling avoids the problem of separating the receptor from the natural environment (such as the cell surface) from other factors.

Publication

Applications of LiGA-based Cell Surface Lectin-binding Profiling

• LiGA-based cell surface lectin-binding profiling enables the analysis of ligand-receptor interactions on living cells and motile surfaces.
• LiGA-based cell surface lectin-binding profiling is an effective tool for analyzing the differences in the glycocalyx composition between normal cells and cancer cells.
• LiGA-based cell surface lectin-binding profiling is essential for discovering lead compounds for next-generation carbohydrate therapies.
• LiGA-based cell surface lectin-binding profiling is a new study target for increasing the immune system or chemotherapy eradication.

CD BioGlyco has established a high-throughput screening Glycan Display Platform. Our talented team will help clients plan the entire program of services. To learn more information about LiGA-based cell surface lectin-binding profiling, please feel free to contact us.