Separation of Glycosaminoglycans

At present, the structure and biological functions of glycosaminoglycans are being extensively studied, and the basis of these analyses is to obtain high-purity glycosaminoglycans. CD BioGlyco has a variety of advanced technology platforms and analysis tools that can help customers study glycosaminoglycans in depth. We have confidence to be your essential research assistant in the field of glycobiology.

Background

Glycosaminoglycan, once called mucopolysaccharide, is mainly found in the connective tissues of higher animals and plants. Glycosaminoglycan (GAG) is a large linear polysaccharide composed of repeating disaccharide units. Its main configuration contains amino sugars (GlcNAc or GalNAc) and uronic acid (glucuronic acid and/or iduronic acid). GAG can be divided into two subgroups: (a) glucosaminoglycans including hyaluronic acid (HA), keratan sulfate (KS), heparan sulfate (HS) and heparin; (b) galactosaminoglycans, which include chondroitin and dermatan sulfate (CS/DS). Except for KS and HA, all GAGs are linked to their parent proteins via xylose-galactose-galactose-glucuronic acid core tetrasaccharide (GlcAb1-3Galb1-3Galb1-4Xylb1-O-Ser).

GAG accounts for a significant portion of glycoconjugates in cell membranes and the extracellular matrix of almost mammalian tissues. Their ability to bind and change protein-protein interactions or enzyme activity significantly affects our understanding of many biological processes, including cell proliferation, differentiation, wound infection and healing. It is also an important cofactor for a variety of cell behaviors.

Fig 1. Monosaccharides in GAGFig 1. Monosaccharides in GAG (Valcarcel, J.; et al. 2017)

Services

We provide services to isolate and purify high-quality GAG from various samples such as cells or tissues and can perform subsequent GAG profiling as needed. The separation service process provided by CD BioGlyco is as follows.

  • The degradation of the core protein must be inhibited before purification. We can take ice excision of the tissue and freeze it quickly to ensure the integrity of the sample. (In the case of extracting membrane-related GAG, tissue-specific pretreatment or detergent is usually added before extraction.)
  • A combination of a variety of separation methods (including organic solvent precipitation, fractionation, ultracentrifugation, ion-exchange chromatography and size-exclusion chromatography, etc.) are used to separate GAG from other proteins. Antibodies are also useful tools for the purification of corresponding GAG.

Applications

  • GAG function and structure analysis
  • Research on the interaction between GAG and ligand
  • Development glycosaminoglycan-based therapeutic
  • Application in cosmetics and health care products

Advantages of Us

  • Capable of processing various types of samples
  • High speed and high selectivity
  • Experienced researchers and trusted results
  • Cost-effective

CD BioGlyco has a lot of successful experience in the separation and profiling of glycosaminoglycans, and our services have helped customers from all over the world, the efficient services and accurate results have gained a good reputation worldwide for our company.

Customers can contact our employees directly and we will respond promptly. If you are interested in our services, please contact us for more detailed information.

Reference:

  1. Valcarcel, J.; et al. Glycosaminoglycans from marine sources as therapeutic agents. Biotechnology Advances. 2017, 35(6): 711-725.
This service is for Research Use Only, not intended for any clinical use.

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CD BioGlyco is a world-class biotechnology company with offices in many countries. Our products and services provide a viable option to what is otherwise available.

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