NGL Immobilization Service

NGL Immobilization Service

Client Satisfaction with NGL Immobilization Service Is Our Goal

CD BioGlyco has extensive experience with NGL display arrays based on the Glycan Display Platform. Immobilization of NGL on solid surfaces or incorporation into liposomal formulations is an important step in the construction of NGL Display Arrays. For this purpose, we have studied the effect of various methods on NGL immobilization. Based on our research experience, we offer high-quality NGL immobilization services. Our goal is to immobilize NGL in liposomal formulation or on solid supports in a controlled and stable manner.

  • Covalent Immobilization

We chemically attach the NGL to the solid support through covalent bonding. Various functional groups on the solid support and NGL are used for the affixation such as amino group and carboxyl group.

  • Non-covalent Immobilization

We also employ non-covalent interactions to immobilize the NGL to the solid support. Common methods include hydrophobic interactions, electrostatic interactions, or specific binding interactions. For example, a lipid anchor with a hydrophobic portion is immobilized by adsorption onto hydrophobic solid support via hydrophobic interactions.

  • Polyvalent Presentation

NGL is used in inhibition assays when doped into liposomes in a multivalent state. We immobilize NGL in a multivalent manner in which multiple copies of NGL are displayed on a solid support. In this way, the affinity and binding of NGL to specific proteins are enhanced. This method also mimics the multivalency present in natural carbohydrate recognition events.

The choice of the NGL immobilization method depends on the structure of the NGL, the solid support material, the desired stability, and the application. During the immobilization process, we optimize and examine the effects of NGL concentration and structure, solid support material, connecting molecules, durability, and other factors. Stable and reliable NGL immobilization is ultimately achieved through continuous optimization.

Fig.1 Methods of NGL immobilization. (CD BioGlyco)Fig.1 Methods of NGL immobilization. (CD BioGlyco)

Publication Data

Technology: Chemical conjugating

Journal: FEBS Letters

IF: 2.675

Published: 2018

Results: This paper describes a method for preparing a microarray of lipid-linked oligosaccharide probes by covalent immobilization. The microarray combines NGLs and oligosaccharide arrays with each other. The microarray was employed to examine the oligosaccharide binding properties of the C-type lectin-like protein dectin-1, and the results showed that dectin-1 binds to 10 aggregates or longer β1,3-linked oligoglucose sugars. This method provides an idea for NGL immobilization and NGL-based analysis.

Fig.2 Detection results of oligosaccharide binding properties of dectin-1 by microarrays based on lipid-linked oligosaccharide probes. (Li, et al., 2018)Fig.2 Detection results of oligosaccharide binding properties of dectin-1 by microarrays based on lipid-linked oligosaccharide probes. (Li, et al., 2018)

Applications of NGL Immobilization

  • Stable and controlled immobilization of NGL on solid support allows researchers to gain insight into the specificity, function, and therapeutic potential of carbohydrate recognition events.
  • Stable and controlled immobilization of NGL on solid support allows for the study of glycan-protein interactions, and drug discovery.
  • Stable and controlled immobilization of NGL on solid support allows the study of the role of carbohydrates in various biological processes.

Advantages of Us

  • Our stable and reliable NGL immobilization service is essential for the creation of NGL display arrays and other platforms for studying protein-carbohydrate interactions.
  • During the immobilization process, we optimize and examine the effects of NGL concentration, stability, durability, and other factors.
  • Our goal is to provide a strong NGL bond that withstands subsequent experimental manipulations, washing steps, etc., without compromising the integrity of the NGL.

CD BioGlyco provides screening services based on the NGL display array with superior technology. To better serve our clients, we have researched experiments related to Artificial Glycolipid Synthesis and NGL immobilization. Please feel free to contact us for more detailed information about NGL fixation methods and experimental optimization.

Reference

  1. Li, Z.; Feizi, T. The neoglycolipid (NGL) technology-based microarrays and future prospects. FEBS letters. 2018, 592(23): 3976-3991.
This service is for Research Use Only, not intended for any clinical use.

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