Analysis of sialic acid is critical due to its identification as a marker of various disease states. CD BioGlyco has developed various strategies to analyze sialic acid, including high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), liquid chromatography-mass spectrometry (LC-MS), fluorescence, specific bio-affinity strategies, metabolic glycan labeling, and sialic acid linkage-specific glycosylation profiling.
Sialic acid is a family of nine carbon carboxylated monosaccharides with high structural diversity. It consists of N-acetylneuraminic acid (Neu5Ac), N-glycolylneuraminic acid (Neu5Gc), deaminoneuraminic acid (KDN), and their derivatives with modifications, such as acetylation, lactylation, methylation, and sulfation at the 4, 7, 8, and 9 positions. Sialic acid exists as either free or bound sugar linked to galactose, N-acetylgalactosamine, and other sugar residues in various positions. Furthermore, sialic acid links to sialic acid itself to form dimeric, oligomeric, and polymeric structures. Thus, sialic acid shows extremely high structural diversity due to monosaccharide species as well as linkage modes. Analysis of sialic acid is critical due to its identification as a marker of various disease states. Therefore, identification and quantification of sialic acid could help in validating sialic acid as a marker for cancers aiding in diagnosis and more rapid treatment. Currently, accurate, simple, and rapid methods for the quantification and analysis of sialic acid have been developed.
HPAEC-PAD is an effective way to quantify sialic acids without derivatization. CD BioGlyco provides accurate, reproducible, and effective analysis of sialic acid using HPAEC-PAD.
CD BioGlyco has developed various fluorimetric methods to detect and quantify sialic acids in samples for our clients worldwide, including fluorometric assays and fluorescence imaging.
Metabolic glycans labeling has emerged as a powerful tool for the analysis of sialylated glycans and glycosylation in living cells and animals. At CD BioGlyco, we label sialylated glycans on the cell surface with Ac4ManNAz.
CD BioGlyco employs various approaches to provide professional and rapid sialic acid analysis services for our clients. For more information, please feel free to contact us.