Chemoenzymatic Synthesis of Glycoproteins

CD BioGlyco has a variety of advanced technology platforms and customized glycoprotein synthesis services. The high-quality samples we provide can perform well in customers' research. We have confidence to be your essential research assistant in the field of glycobiology.

Background

Glycosylation is one of the most common post-translational modifications, which profoundly affects the structure and function of proteins. Most therapeutic proteins (including monoclonal antibodies) are glycosylated, and the presence and fine structure of sugar chains are critical to the stability and biological function. Therefore, understanding the structure and function as well as the control of glycosylation status is essential for the development and production of protein-based therapeutics. However, the structural heterogeneity of natural and recombinant glycoproteins caused by changes in glycan composition and glycosylation sites complicates the in-depth study of their structure-function relationships and hinders the applications in the field of biotherapy.

Recombinant glycoproteins such as therapeutic antibodies usually consist of a mixture of glycoforms with the same protein backbone but differ in the pendent oligosaccharides. It is difficult to separate completely homogeneous glycoforms using current chromatographic techniques. In recent years, tremendous progress has been made in the production of homogenous glycoproteins, including tailormade glycoforms of intact antibodies. Those include total chemical synthesis, "tag and modify" approaches for site-selective protein-glycan conjugation, chemoenzymatic synthesis, and glycosylation chemical engineering by the manipulation of biosynthetic pathways in different host expression systems.

Fig 1. Overview of glycoprotein synthesisFig 1. Overview of glycoprotein synthesis (Li, C.; Wang, L.X. 2018)

Services

At CD BioGlyco, we deal with three major tasks when chemically and enzymatically synthesizing homogeneous glycoproteins: the production of polypeptide or protein backbone; the preparation or enrich of oligosaccharide/glycan component; and the conjugation of glycan to the protein/polypeptide backbone. Compared with chemical synthesis, enzymatic and chemoenzymatic synthesis is an attractive and emerging method, which greatly complements and expands the chemical method of constructing homogeneous glycoproteins. We provide chemoenzymatic synthesis methods using the following technical routes, and we can also customize the synthesis scheme according to the research needs of customers.

  • The endoglycosidase-catalyzed convergent glycosylation for N-glycoprotein synthesis and glycan remodeling
  • The direct enzymatic glycosylation of intact proteins
  • The post-translational glycan modifications of glycoproteins

Applications

  • Glycoproteins structure & function research
  • Development of therapeutic glycoproteins
  • Research on glycoprotein-related cellular activities

Advantages of Us

  • Multiple advanced chemoenzymatic synthesis technology platforms
  • Various modifications can be customized according to needs
  • Able to synthesize complex glycoproteins
  • 24 hours after-sales service

CD BioGlyco has many years of successful experience in glycoproteins synthesis. Our professional and rigorous synthetic chemists can provide customers with high-quality and homogeneous glycoforms glycoproteins.

Customers can contact our employees directly and we will respond promptly. If you are interested in our services, please contact us for more detailed information.

Reference:

  1. Li, C.; Wang, L.X. Chemoenzymatic methods for the synthesis of glycoproteins. Chem Rev. 2018, 118(17): 8359-8413.
This service is for Research Use Only, not intended for any clinical use.

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About Us

CD BioGlyco is a world-class biotechnology company with offices in many countries. Our products and services provide a viable option to what is otherwise available.

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