MOS are non-digestible oligosaccharides derived via partial hydrolysis of the mannans polysaccharide, which can be generally divided into two main groups: α-and β-MOS. While α-MOS are obtained by cleavage of α-(1-6) bonds from yeast cell wall mannans, β-MOS are commonly obtained from mannans-rich plants through cleavage of β-(1-4)-glycosidic bonds.
Fig.1 The structure of MOS. (Wikipedia)
The α-(1-3) and α-(1-6) branched of MOS present in the cell wall of S. cerevisiae are particularly effective at binding pathogens. When the attachment and colonization of the intestine by gram-negative pathogenic bacteria is blocked with MOS, the autogenous population can flourish, making the gut more efficient, and liberating more nutrients for lean tissue growth and improving immunity in the host animal. However, as reported in the studies, the MOS effect on promoting beneficial bacteria, such as Lactobacilli and Bifidobacteria is more variable.
Fig.2 Potential action mechanisms of MOS in pathogen colonization inhibition. (Faustino, et al., 2021)
CD BioGlyco offers various Oligosaccharides Analysis Services to meet the specific requirements of our global clients with multiple advanced technology means. We are looking forward to being your research assistant in the field of oligosaccharides analysis.
Our MOS analysis process is as follows:
Isolation of mannan: The sample is crumbled into a citrate buffer solution, and the mixture is autoclaved. Crude mannan is obtained and purified, and then directly weighed.
Acetolysis of mannan and isolation of mannose oligosaccharides: The reaction products are extracted with chloroform.
HPLC, MALDI-TOF-MS, and NMR analysis are used to analyze the structure of MOS.
Fig.3 The structure analysis of MOS. (CD BioGlyco)
CD BioGlyco provides accurate, reliable, and reproducible MOS analysis services for our clients worldwide. If you are interested in our services, please feel free to contact us.