Based on our complete carbohydrate metabolism analysis solutions, CD BioGlyco provides xylose analysis services for our clients worldwide through UV-visible spectrophotometry, high-performance liquid chromatography (HPLC), and high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD).
Xylose is an aldopentose-type monosaccharide obtained from hemicellulose and was first isolated from wood by Finnish scientist Koch in 1881. It is by far the most abundant pentose sugar and can be converted into ethanol, furfural, and xylitol. At present, the use of xylose to produce ethanol is a global research hotspot. If xylose is used as a carbon source in fermentation, the energy and food crisis can be solved. Therefore, researchers focused on developing efficient xylose-fermenting microorganisms for bioethanol production. Xylitol is a polyol widely used in food, cosmetics, and pharmaceutical additives, which can be obtained by catalytic hydrogenation of xylose.
Fig.1 Xylose structure. (Wikipedia)
In the body, xylose is converted to xylulose in two ways: 1) Xylose reductase reduces xylose to xylitol, which is then converted to xylulose by the action of xylitol dehydrogenase; 2) In some bacteria and lower fungi, xylose metabolism is initiated by xylose isomerase, which converts xylose directly to xylulose. Xylulose is phosphorylated under the action of xylulose kinase to form xylulose-5-phosphate. Xylulose-5-phosphate begins to enter the pentose phosphate pathway (PPP) to produce ethanol. At present, the difficulty in utilizing xylose for many microorganisms is the transportation of xylose and the conversion of xylose to xylulose. Therefore, researchers have tried various genetic engineering strategies aimed at converting xylose to xylulose, making it available for PPP.
Fig.2 Xylose metabolism in microorganisms. (Sheet, 2014)
CD BioGlyco has been focusing on carbohydrate research for many years and has developed complete Carbohydrate Metabolism Analysis solutions. We provide xylose analysis services in cereals, fermentation broth, and more for our clients worldwide. Our methods include:
The xylose content in the fermentation samples can be determined according to the linear relationship between the xylose concentration and the absorbance.
HPLC is the most common chromatographic technique for the determination of sugar content. We can determine xylose content in samples using sensitive, selective, and reliable HPLC methods with an evaporative light scattering detector (ELSD) or refractive index detector (RID).
HPAEC-PAD can be used to determine xylose content in cereals. It has the advantage of high sensitivity.
CD BioGlyco is a leader in carbohydrate research, providing sensitive, selective, and reliable xylose analysis services for our global clients. If you are interested in our services, please feel free to contact us, we are looking forward to being your research assistant in the field of carbohydrates.