Mannan-binding Lectin Production Service

Mannan-binding Lectin Production Service at CD BioGlyco

CD BioGlyco offers one-stop services for Recombinant Lectin Production using our advanced Lectin expression platform technologies. Our full services include protein gene synthesis, protein expression vector design, large-scale cell culture and fermentation, Protein Purification, and protein quality control testing. We offer significant record speed in lectin expression and bulk production with extensive experience and expertise.

Mannan-binding Lectin

Mannose-binding protein (MBP) or lectin (MBL) is a lectin that acts as an adsorbent in innate immunity and through the lectin-complement pathway. MBL consists of subunits with an oligomeric structure (400-700 kDa) containing three probably identical peptide chains of approximately 30 kDa.

Fig.1 Structure of the MBL. (Wikipedia)

CD BioGlyco offers two types of recombinant mannan-binding lectin custom production services.

α-Mannose-binding Lectin Production Service

With our expertise and experience in recombinant lectin production, we provide α-mannose-binding lectin production service.

α-Mannose, Lewis a (Lea) & Lewis x (Lex)-binding Lectin Production Service

CD BioGlyco offers α-Mannose, lewis a (Lea) & elwis x (Lex)-binding lectin production service. Our experienced research team is able to tailor solutions to the specific needs of our clients.

Fig.2 Steps for a lectin production. (CD BioGlyco)

Publication

Technology: Gel-filtration Chromatography

Journal: Marine Biotechnology

IF: 3.152

Published: 2013

Results: In this study, a mannan-specific lectin ((MBL-SN)) was isolated from the sea urchin plasma using a combination of gel filtration chromatography, affinity chromatography, and ion exchange chromatography. The physicochemical characterization showed that the molecular weight of the lectin was about 34 kDa, and it belonged to the C-type mannose-specific lectin. It had a common folding pattern compared to the existing three-dimensional structure of C-type mannose-specific lectins.

Fig.3 The 3D structure model of MBL-SN. (Bulgakov, et al., 2013)

Applications

As Receptors for Receptor-Mediated Targeting

MBL is a circulating protein that functions as a soluble pattern-recognition molecule in innate immunity.
MBL spots high-mannose forms of glycans in foreign microorganisms or plant pests.
MBL distinguishes carbohydrate structures expressed on the surfaces of multiple bacteria; MBL triggers carbohydrate-mediated complement activation.
MBL has complement-dependent bactericidal action.

Healing Applications of MBL

MBL replacement strategy has been applied to treat radiation-induced chronic ulcers.
MBL is shown to have a major impact on the complement system in the remodeling process of bone healing and in the pathophysiology of diabetic nephropathy.

Advantages

Over years of experience in recombinant lectins production.
One-stop customized service from gene synthesis and vector construction to lectin expression and purification.
We offer multiple lectin expression systems to choose from based on your special needs, including baculovirus-insect, yeast, bacterial, and mammalian expression systems.
High R&D ability and quick problem-solving ability.
We guarantee high-throughput and large-scale production.

With strong expertise and experience in recombinant lectin production, CD BioGlyco offers rapid and efficient mannan-binding lectin production services. We offer one-stop services for recombinant lectin production using our advanced protein expression platform technologies. Please feel free to contact us if you have any questions about the mannan-binding lectin production program.

Reference

Bulgakov, A.A.; et al. Mannan-binding lectin of the sea urchin Strongylocentrotus nudus. Marine Biotechnology. 2013, 15: 73-86.

This service is for Research Use Only, not intended for any clinical use.