There is a close link between tumor tissue hypoxia and reprogramming of glucose metabolism. Tumor tissue hypoxia can promote tumor invasion, migration, angiogenesis, and other malignant phenotypes. Detection of tumor tissue hypoxia is of great significance for the study of tumorigenesis and development mechanism and the evaluation of targeted drugs. CD BioGlyco provides tumor tissue hypoxia detection services by detecting HIF protein expression and other hypoxia markers. Because HIF has a short half-life and is sensitive to oxygen levels, it is difficult to detect it, so sometimes the key proteins regulated by HIF are selected to be detected to reflect tissue hypoxia.

Fig.1 The detection of hypoxia in vivo (Godet I, et al., 2022)

What We Do

There are various methods for the detection of hypoxia in tumor tissue, we can provide the following specific services:

• Immunolabeling of endogenous hypoxia markers
• Detection of tumor tissue hypoxia by intravenous injection of exogenous hypoxia probe
• Hypoxia-dependent fluorescent reporter gene analysis
• Fluorescent molecules activated by expression of nitroreductive molecules to detect hypoxia in tumor tissue
• Hypoxia-dependent bioluminescence reporter gene analysis
• Molecular imaging analysis of O₂-quenched phosphorescence in mice
• Changes in the ratio of oxyhemoglobin and deoxyhemoglobin to protein detected by functional magnetic resonance imaging
• Choosing an appropriate method to detect tumor tissue hypoxia requires attention to whether the chosen method is suitable for in vivo and the sensitivity and scale of the measurement.

Detection Principle

We use immunological methods to detect hypoxia in tumor tissue. First, we injected mice with 2-nitroimidazole-like probes. Under hypoxic conditions, these probes form stable adducts with sulfhydryl groups in proteins, polypeptides, and amino acids. Such probes selectively accumulate in hypoxic cells and then detect hypoxia in tumor tissue through antibody tags that can specifically recognize these adducts.

Applications

• The prognostic value of hypoxia in tumor tissue.
• Hypoxia up-regulation of markers of cancer metabolism, migration, and chemoresistance.
• The role of cancer cells undergoing hypoxia in the malignant phenotype of tumors.
• Changes in glucose metabolism of cancer cells after hypoxia and reoxygenation.
• Research on hypoxia-targeted therapy.

Notes

• When the probe is injected into mice by intravenous, intraperitoneal, or oral route, the dose is 60 mg/kg.
• The probe can be distributed to all tissues including the brain and forms adducts with protein sulfhydryl groups in cells with oxygen concentration pO₂=10 mm.

CD BioGlyco provides tissue hypoxia detection services. Our experienced teams of scientists, researchers, and technicians provide fast turnaround, high-quality services at competitive prices for worldwide customers. Our customers have direct access to our staff and prompt feedback on their inquiries. If you are interested in our services, please contact us for more details.

Reference:

1. Godet I, et al. Detection of Hypoxia in Cancer Models: Significance, Challenges, and Advances. Cells. 2022 Feb 16; 11(4): 686.