There is a close link between tumor tissue hypoxia and reprogramming of glucose metabolism. Tumor tissue hypoxia can promote tumor invasion, migration, angiogenesis, and other malignant phenotypes. Detection of tumor tissue hypoxia is of great significance for the study of tumorigenesis and development mechanism and the evaluation of targeted drugs. CD BioGlyco provides tumor tissue hypoxia detection services by detecting HIF protein expression and other hypoxia markers. Because HIF has a short half-life and is sensitive to oxygen levels, it is difficult to detect it, so sometimes the key proteins regulated by HIF are selected to be detected to reflect tissue hypoxia.
Fig.1 The detection of hypoxia in vivo (Godet I, et al., 2022)
There are various methods for the detection of hypoxia in tumor tissue, we can provide the following specific services:
We use immunological methods to detect hypoxia in tumor tissue. First, we injected mice with 2-nitroimidazole-like probes. Under hypoxic conditions, these probes form stable adducts with sulfhydryl groups in proteins, polypeptides, and amino acids. Such probes selectively accumulate in hypoxic cells and then detect hypoxia in tumor tissue through antibody tags that can specifically recognize these adducts.
CD BioGlyco provides tissue hypoxia detection services. Our experienced teams of scientists, researchers, and technicians provide fast turnaround, high-quality services at competitive prices for worldwide customers. Our customers have direct access to our staff and prompt feedback on their inquiries. If you are interested in our services, please contact us for more details.