Neutral Lipid Analysis

Neutral Lipid Analysis

CD BioGlyco provides one-stop neutral lipids (NLs) analysis service by high-phase liquid chromatography (HPLC), including standard calibration, lipid extraction, and HPLC analysis.

Introduction of NLs

Fatty acids (FAs) and fatty alcohols are stored in the form of NLs, such as cholesterol ester (CE), triacylglycerol (TAG), and wax ester (WE), which provide organisms with an inert form that are used under conditions of nutrient deficiency and environmental stress. TAG is a fatty acyl ester derivative of glycerol, which represents the main energy store of all eukaryotic cells and some bacterial cells. Among algae, plankton, and bacteria, WE is also used as energy storage. In plants and insects, WE provides a hydrophobic coating of tissue to minimize surface dehydration.

NLs are a heterogeneous array of different alcohols (sterols, diglycerides, long-chain alcohols) combined with FAs. The degree of saturation, chain length, cyclic or linearity of NLs are manifold and may have significant characteristics and functions. Increased cytoplasmic deposition of NLs (mainly CE and TAG) is an important risk factor for the pathology of many diseases, including diabetes, obesity, atherosclerosis, and non-alcoholic fatty liver. For example, the accumulation of CE in smooth muscle cells and macrophages in blood vessel walls constitutes the earliest recognizable stage of atherosclerotic plaque formation. In addition, the serum levels of TAG and total cholesterol are independent risk factors for atherosclerosis.

Metabolic pathway of TAG and sterol ester (SE) synthesis.Fig.1 Metabolic pathway of TAG and sterol ester (SE) synthesis. (Onal, 2017)


Excessive NLs are closely related to a variety of human diseases (such as diabetes, obesity, atherosclerosis, and non-alcoholic fatty liver disease). Therefore, the qualitative and quantitative analysis of NLs may help humans better prevent many diseases. At CD BioGlyco, we analyze NLs by high-phase liquid chromatography (HPLC). The specific analysis steps are as follows:

  • Standard calibration

Dissolve the standards in chloroform, prepare different concentration gradients, and establish an external calibration curve for lipids after injection.

  • Lipid extraction

According to the Folch method (chloroform: methanol, 2:1, v/v) to extract lipids in the sample.

  • HPLC analysis

The HPLC is connected to an evaporative light scattering detector (ELSD) detector for the analysis of NLs.

Applications of NL Analysis

  • Study the mechanism of NLs
  • Early screening of disease
  • Monitor the content of NLs in food
  • Analyze lipids in microorganisms

Advantages of Us

  • High efficiency and high sensitivity
  • High accuracy
  • Complete experiment process and analysis report
  • One-stop analysis solution

CD BioGlyco has always adhered to the customer-centric principle and developed a set of Lipid Analysis solutions. We have already had many successful analysis cases and won unanimous praise from customers. If you have neutral lipid analysis needs, please feel free to contact us.


  1. Onal, G.; et al. Lipid droplets in health and disease. Lipids in Health & Disease. 2017, 16(1): 1-5.
  2. Athenstaedt, K.; Daum, G. The life cycle of neutral lipids: synthesis, storage and degradation. Cellular & Molecular Life Sciences Cmls. 2006, 63(12): 1355-1369.
This service is for Research Use Only, not intended for any clinical use.

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