Fucoidan is chemically a high molecular polysaccharide consisting mainly of sugar called "sulfated fucose". Fucoidan is mainly found in the cell wall matrix and is a resistant and barrier component. The structure of fucoidan is complex, and the components and ratios of fucoidan extracted from different brown algae by different extraction methods are different. After research, it was found that the main constituent unit of fucoidan is 1, 2-α-fucose or 1, 3-α-fucose. Research shows that fucoidan has a variety of biological activities. For example, anticoagulant, antitumor, antiviral, hypolipidemic, and immunity-enhancing pharmacological activities.
Fig.1 The metabolic pathway of fucoidan. (Chao, et al., 2018)
CD BioGlyco has been involved in Polysaccharide Analysis for many years, so we offer our clients a full range of fucoidan analysis services. The analytical testing services we provide for fucoidan are:
Fig.2 Analysis procedure of fucoidan. (CD BioGlyco)
Paper Title: Fucoidans from brown alga Fucus evanescens: structure and biological activity.
Technology: 1H, 13C, and 2D (COSY, TOCSY, ROESY, NOESY, HSQC, and HMBC) nuclear magnetic resonance (NMR) spectroscopy
Journal: Frontiers in Marine Science
Results: The structures of the obtained modified fucoidans were studied by 1D (1H, 13C) and 2D (COSY, TOCSY, ROESY, NOESY, HSQC, and HMBC) nuclear magnetic resonance (NMR) spectroscopy and methylation analysis. As a result, fucoidan was shown to be a regular molecule containing disaccharide repeating units, with a linear main chain of alternating 2-sulfated 1,3-and 1,4-linked α- L-fucose residues. A methylation analysis of desulfated fucoidan showed that 1,3-and 1,4-linked fucose residues had a ratio of 1.2:1. Few sulfate groups were found in position 4 of some 1,3-linked fucose residues. Acetyl groups occupied the free C-3 of 1,4-linked residues and/or the C-4 of 1,3-linked fucose residues (Fig.3).
Fig.3 Structures of fucoidan from F. evanescens. (Menshova, et al., 2016)
As a recognized expert in the field of polysaccharide analysis, CD BioGlyco has developed a complete workflow for fucoidan analysis and has performed many fucoidan analysis projects. Please feel free to contact us for any of your needs in this area.