CD BioGlyco has extensive experience in the synthesis of custom complex N-linked Oligosaccharides. The unique technology platforms and professional researchers of our company ensure that we offer high-efficient services at a competitive price for our clients. We have the confidence to help our clients accelerate their research on glycobiology by reducing project costs and timelines.

Background

N-linked oligosaccharide is characterized by the attachment of glycans to an asparagine side chain of the protein. The glycosylation site of N-linked oligosaccharides is Asn-X-Ser/Thr, where X represents any amino acid except proline. N-linked oligosaccharides have important biological significance, including enhancing solubility and stability of protein, acting as signal molecules in protein folding and transportation, determining the specificity of membrane receptors in cell-to-cell interactions or innate immunity, and changing the properties of hormones and secreted proteins.

The biosynthesis of N-linked glycans occurs through 3 major steps: synthesis of dolichol-linked precursor oligosaccharides, en bloc transfer, and initial trimming of precursor oligosaccharides in the endoplasmic reticulum (ER), and Subsequent processing in the Golgi apparatus. At present, multi-step Chemical or biological synthesis of N-linked oligosaccharides with various structures is the main effective way to obtain N-linked oligosaccharides by artificial strategy, which is of great significance to study the relationship between saccharides and disease.

Fig.1 Different stages of N-glycan complexity. (Fisher, 2016)

Services

Equipped with advanced technology platforms and professional research teams in the field of glycobiology, CD BioGlyco has established effective chemical or chemoenzymatic methods to synthesize high-purity N-linked oligosaccharides for our customers. The services we provide include but are not limited to:

• Characterization and profiling of N-linked oligosaccharides.
• Synthesis of various N-linked oligosaccharides by effective and concise chemical methods.
• Synthesis of complex N-linked oligosaccharides through the chemoenzymatic method according to clients’ demands.
• Various modifications of N-linked oligosaccharides according to customers’ scientific requirements.
• Purity and quality detection of customized synthesized N-linked oligosaccharides.

Applications

• Research of functional N-linked oligosaccharides
• Research of interaction between N-linked oligosaccharides and protein
• Research on the relationship between N-linked oligosaccharides and diseases

Advantages of us

• Extensive experience in complex N-linked oligosaccharides synthesis
• Optimized experimental conditions
• Highly flexible multi-step synthesis method.
• Products with high yield and high stereoselectivity

CD BioGlyco has successfully synthesized a variety of complex N-linked oligosaccharides through multi-step synthesis. We have the confidence to offer custom complex types of N-glycans synthesis service with high quality for our clients. If you are interested in our services, please contact us for more detailed information.

References:

1. Mitra, N.; et al. N-linked oligosaccharides as outfitters for glycoprotein folding, form and function. Trends in Biochemical Sciences. 2006, 31(3):156-163.
2. Fisher, P.; Ungar, D. Bridging the gap between glycosylation and vesicle traffic. Frontiers in cell and developmental biology. 2016, 4:15.