CD BioGlyco has extensive experience in the synthesis of custom complex N-linked Oligosaccharides. The unique technology platforms and professional researchers of our company ensure that we offer high-efficient services at a competitive price for our clients. We have the confidence to help our clients accelerate their research on glycobiology by reducing project costs and timelines.
N-linked oligosaccharide is characterized by the attachment of glycans to an asparagine side chain of the protein. The glycosylation site of N-linked oligosaccharides is Asn-X-Ser/Thr, where X represents any amino acid except proline. N-linked oligosaccharides have important biological significance, including enhancing solubility and stability of protein, acting as signal molecules in protein folding and transportation, determining the specificity of membrane receptors in cell-to-cell interactions or innate immunity, and changing the properties of hormones and secreted proteins.
The biosynthesis of N-linked glycans occurs through 3 major steps: synthesis of dolichol-linked precursor oligosaccharides, en bloc transfer, and initial trimming of precursor oligosaccharides in the endoplasmic reticulum (ER), and Subsequent processing in the Golgi apparatus. At present, multi-step Chemical or biological synthesis of N-linked oligosaccharides with various structures is the main effective way to obtain N-linked oligosaccharides by artificial strategy, which is of great significance to study the relationship between saccharides and disease.
Fig.1 Different stages of N-glycan complexity. (Fisher, 2016)
Equipped with advanced technology platforms and professional research teams in the field of glycobiology, CD BioGlyco has established effective chemical or chemoenzymatic methods to synthesize high-purity N-linked oligosaccharides for our customers. The services we provide include but are not limited to:
CD BioGlyco has successfully synthesized a variety of complex N-linked oligosaccharides through multi-step synthesis. We have the confidence to offer custom complex types of N-glycans synthesis service with high quality for our clients. If you are interested in our services, please contact us for more detailed information.