LBP is the main active substance in L. barbarum. LBP is a complex mixture of polysaccharides affixed with a wide range of biological activities. Several experiments have proved that LBP has antioxidant activity, etc. The thymus is a central immune organ that primarily mediates cellular immunity, and the spleen is a secondary immune organ involved in humoral immunity. LBP has shown significant improvement in the thymus index and spleen index of model animals with severe aging. Meanwhile, LBP can increase the abundance and diversity of intestinal microbiota, maintain the balance of intestinal microbiota, and improve the intestinal barrier to play a hepatoprotective role. It can regulate the PI3K/Akt/mTOR signaling pathway and finally induce apoptosis in A549 cancer cells.
CD BioGlyco develops a series of analytical services around the focus of Carbohydrate Metabolism concerns. Analyzing the structure of LBP is the basis for LBP structure-efficacy relationship studies. It is important for in-depth exploration of the mechanism of action of its physiological functions, as well as in-depth utilization and development of LBP. We provide LBP structure analysis and bioactivity analysis services.
We provide LBP structural characterization services, including relative molecular mass, monosaccharide composition, functional groups, types of glycosidic bonds and connections, etc. LBP structure mostly carries non-saccharide modifications (methylation, carboxymethylation, acetylation, etc.). Under the existing conditions, it is difficult to complete the complete structural characterization of LBP by a single method. Therefore, we combine multiple methods for comprehensive characterization. We apply new methods and techniques such as mass spectrometry (MS), nuclear magnetic resonance (NMR), and glycomics to the structural analysis of LBP. We also combine traditional methods of chemical structure analysis of glycans such as methylation, periodate oxidation, and Smith degradation. Among them, we are experienced in glycosidic bonding and conformation. We mainly use methylation reactions, partial acid or enzymatic hydrolysis, and NMR to determine sugar conformations, glycosidic bond types, and sugar residue attachment sites. We apply high-performance anion exchange chromatography (HPAEC) to determine the monosaccharide composition of LBP. The method is highly sensitive. No derivatization of the sample is required.
Fig.1 LBP resolution program. (CD BioGlyco)
Studies have shown that LBP has antioxidant, anti-aging, anti-tumor, anti-fatigue, anti-radiation, hepatoprotection, etc. We are experienced in cell culture and mouse model construction. We will complete any bioactivity assay experiments according to clients' requirements.
Paper Title: Lycium barbarum polysaccharides improve lipid metabolism disorders of spotted sea bass Lateolabrax maculatus induced by high lipid diet
Technology: Gel permeation chromatography coupled with multi-angle laser light scattering (GPC-MALLS)
Journal: International Journal of Biological Macromolecules
Results: In this study, the molecular weight of LBP was determined by GPC-MALLS. The monosaccharide composition of each component was analyzed by an ion chromatography system and electrochemical detector. Two compounds with molecular weights of 11.343 and 35.508 kDa were isolated from LBP. The monosaccharide compositions of the two compounds were different. LBP was able to ameliorate lipid metabolism disorders and enhance the antioxidant capacity of spotted sea bass.
Fig. 2. Molecular conformation plot of two LBP compositions. (Huang, et al., 2023)
CD BioGlyco is committed to facilitating research related to carbohydrate metabolism. In addition to LBP, we also provide Glycogen and Glucomannan Analysis Services. The services we provide are validated by years of market practice and can help you improve efficiency and reduce costs. Feel free to contact us for more detailed information on polysaccharide analysis.