CD BioGlyco is at the forefront of glycobiology, offering a cutting-edge glycan display platform and comprehensive services that empower our clients to explore, analyze, and manipulate glycans with precision and flexibility for applications such as drug development.

Comprehensive and Custom Glycan Display Service at CD BioGlyco

Our glycan display platform stands out for its versatility, offering traditional glycan display, cell-based glycan display, neoglycolipid (NGL) display, liquid glycan display (LiGA), glyco-phage display, and de novo glycan display methodologies. Our service advantages lie in providing tailored solutions for glycobiology research, spanning drug discovery, biosensor development, and glycan-protein interaction studies. With a commitment to excellence, we collaborate closely with clients, offering a platform for advancing their specific research goals.

Traditional Glycan Display Array

At CD BioGlyco, our traditional glycan display array services encompass Natural oligosaccharide library construction, Chemical and Enzymatic synthesis-based oligosaccharide library construction, Modular synthesis-based oligosaccharide library construction, oligosaccharide library Immobilization, Glycan Microarray Assay, and more. These services provide researchers with a comprehensive toolkit to explore diverse glycan structures and interactions, ultimately advancing glycobiology research.

Fig.1 The standard process of the traditional glycan display array. (CD BioGlyco)

Our glycan microarray assay includes but is not limited

• Synthetic Glycan Microarray Assay
• Microbial Glycan Microarray (MGM) Assay
• Human Milk Oligosaccharide (HMO) Microarray Assay
• Sialylated Oligosaccharides Microarray Assay
• Glycosaminoglycan (GAG) Microarray Assay
• ABH Blood Type I-VI Antigen Microarray Assay

Cell-based Glycan Display Array

CD BioGlyco provides the cell-based glycan display array, leveraging cutting-edge service technologies to delve into the intricate interplay of glycans within cellular environments. Our advanced offerings encompass precise Glyco-engineered Cell Construction, and the development of cell-based O-Glycan, N-Glycan, GAG, and Glycoprotein arrays.

Through a streamlined service process, we apply sophisticated techniques in customized cell engineering design, ensuring accurate glycan presentation. Meticulous construction is followed by rigorous quality control, validating glycan structures and array functionality. This technologically driven approach empowers researchers to unravel the complexities of glycans at the cellular level, propelling glycobiology research to new frontiers. Below are our custom cell construction services.

• GPI Anchor Pathway-based Glyco-engineered Cell Construction Service
• Glycolipids (GSL) Pathway-based Glyco-engineered Cell Construction Service
• N-linked Glycan Pathway-based Glyco-engineered Cell Construction Service
• O-GalNAc Pathway-based Glyco-engineered Cell Construction Service
• O-Fuc Pathway-based Glyco-engineered Cell Construction Service
• O-Man Pathway-based Glyco-engineered Cell Construction Service
• C-Man Pathway-based Glyco-engineered Cell Construction Service
• O-Glc Pathway-based Glyco-engineered Cell Construction Service
• O-Xyl Pathway-based Glyco-engineered Cell Construction Service
• O-Gal Pathway-based Glyco-engineered Cell Construction Service
• O-GlcNAc Pathway-based Glyco-engineered Cell Construction Service
• Glycan Elongaton/branching Pathway-based Glyco-engineered Cell Construction Service
• Glycan Capping Pathway-based Glyco-engineered Cell Construction Service

Neoglycolipid (NGL) Display Array

CD BioGlyco presents the NGL display array, a specialized service catering to the exploration of glycans in unique lipid contexts. This offering enables researchers to study glycan-lipid interactions and their implications across diverse applications. Our service encompasses the creation of tailored NGL display arrays, providing a platform for in-depth investigations into glycan-lipid interplay. Through advanced methodologies, we ensure precise and reliable presentation of neoglycolipids, empowering researchers to unlock new insights in glycobiology. With CD BioGlyco's expertise, the NGL display array becomes a valuable tool for unraveling the intricate roles of glycans in lipid-based environments.

• Artificial Glycolipid Synthesis Service
• NGL Immobilization Service

Fig.2 Schematic representation of the NGL-based microarray system. (Palma, et al., 2014)

Liquid Glycan Display Array (LiGA)

CD BioGlyco introduces the LiGA, a cutting-edge service designed to unravel the complexities of glycans in liquid environments. This innovative offering provides researchers with a versatile platform for studying glycan interactions and functions in a fluidic context. Our LiGA service involves the construction of dynamic glycan arrays, allowing for high-throughput screening and in-depth analysis. With precision and adaptability, CD BioGlyco empowers researchers to explore liquid-phase glycan dynamics, advancing the understanding of glycobiology.

Here are the LiGA services you can find at CD BioGlyco

• LiGA Construction Service
  • Conjugation Strategy for Glycan Linking to Coat Protein pVIII
  • Engineered M13 Bacteriophage Construction Service
• LiGA-based Glycan Modification Service
• Quality Control of LiGA
• Functional Validation of LiGA
  • LiGA-based Glycan-binding Profiling
  • LiGA-based Cell Surface Lectin-binding Profiling
  • LiGA-based Glycan Binding Response Profiling

Fig.3 LiGAs identify the glycoconjugates with optimal avidity necessary for binding to lectins. (Sojitra, et al., 2021)

Glycophage Display

CD BioGlyco spearheads glycophage display technology with a range of specialized services. Our offerings include N-linked and O-linked glycoprotein glycophage display system construction, glycophage display-based Glycosylase Genetic Analysis, Glycoarray, Antibody Development, Epitope Mapping, and Biomarker Development. Through advanced service technologies, we meticulously construct tailored glycophage display systems, enabling high-precision glycan analysis, array development, antibody generation, epitope mapping, and biomarker discovery.

• N-linked Glycoprotein-based Glycophage Display System
• O-linked Glycoprotein-based Glycophage Display System

De Novo Glycan Display

De novo glycan display facilitates the elucidation of glycan structures, including the sequence and linkage information of monosaccharides within complex glycans. This is crucial for understanding the biological functions of glycans in various physiological and pathological processes.

Here are the de novo glycan display services you can find at CD BioGlyco.

• Cell-surface Glycan Editing
• Examining the Effects of Altering Blood Group Antigens on Erythrocyte Cell Surfaces
• Preventing Cellular Rejection During Transplantation
• Modulating Chemical Composition and Physical Parameters of Glycocalyx
• Tailoring Cell Membranes with Biologically Active Glycans
• Targeting Glycosaminoglycan-mediated Growth Factor Signaling to Influence Stem Cell Specification
• Long-term De Novo Glycan Display for Directing Stem Cell Fate

Publication

Applications

• Facilitating high-throughput screening to identify glycans with specific interactions, aiding in the discovery of potential therapeutic candidates.
• Contributing to the creation of innovative biosensors for real-time monitoring of glycan interactions, enabling sensitive and specific detection methods.
• Providing a platform for in-depth studies on glycan-protein interactions, unraveling the complexities of biological processes and potential therapeutic targets.
• Allowing the assembly of glycan libraries with distinct DNA-encoded barcodes, providing researchers with a comprehensive toolkit for studying a variety of glycan structures.

Highlights

• Advanced glycan display platform

Access to a cutting-edge glycan display platform that supports diverse methodologies, such as traditional glycan display, cell-based glycan display, NGL display, LiGA, glycophage display, and de novo glycan display.

• Customized solutions

Tailored services that allow researchers to customize experiments based on specific project requirements, providing flexibility in addressing unique research objectives.

• Comprehensive glycan library creation

Expertise in creating glycan libraries with distinct DNA-encoded barcodes, enabling researchers to explore a wide range of glycan structures for various applications.

At CD BioGlyco, our advanced glycan display platform and suite of services empower clients across diverse applications. From facilitating high-throughput screening for drug discovery to enabling precise glycan engineering for therapeutic proteins, our platform serves as a versatile tool. Additionally, it contributes to the development of innovative biosensors, aids in glycan-protein interaction studies, and offers tailored solutions for a wide range of research needs, further solidifying its pivotal role in advancing glycobiology research and applications. Please do not hesitate to contact us for more details if you are interested in our services and advanced platform.