The pentose phosphate pathway is recognized as a major regulatory pathway for cellular biosynthetic metabolism and control to maintain cellular redox homeostasis. The PPP starts with a product of glycolytic metabolism and is divided into two stages, metabolic intermediates include 6-phosphoglucolactone, NADPH, 5-phosphoribulose, 5-phosphoribose, fructose-6-phosphate, and glycero-3 carbonic acid. PPP metabolites play an important role in the reprogramming regulation of glucose metabolism in cancer. CD BioGlyco can provide targeted metabolomic analysis services for the PPP. Samples we can provide testing services include plasma or serum, urine, saliva, cell, and tissue extracts. Typically, we use nuclear magnetic resonance spectroscopy, mass spectrometry, liquid chromatography-mass spectrometry, gas chromatography-mass spectrometry, and capillary electrophoresis-mass spectrometry for metabolomic analysis.

Our Workflow

Fig. 1 The workflow of targeted metabolomics analysis

What We Do

• Quantitative and qualitative analysis of PPP metabolites

Based on our MOPCGM platform, quantitative and qualitative analysis of PPP metabolites under different treatment conditions by targeted metabolomics is the cornerstone of conducting PPP research.

• PPP metabolites sensing and signaling analysis

Glucose metabolites are not only metabolites but also can be used for signal analysis to regulate cell signal transduction. Reprogramming of glucose metabolism reprograms the metabolic network of cancer leading to dysregulation of glucose metabolites, while cancer cells are able to adapt to changes in glucose metabolites to coordinate multiple biological processes in cancer progression. Therefore, studying the role of PPP metabolite signaling can provide insight into the process of glucose metabolism reprogramming and provide new opportunities for the development of cancer treatment strategies.

• PPP metabolites binding protein analysis

The essence of life activities comes from the complex interactions and chemical reactions between biomolecules in the body. The small molecule metabolites of glucose metabolism can be linked to proteins and play an important role in regulating the function of proteins. We are able to provide an analysis of interactions between PPP metabolites and proteins.

We can provide the following specific services.

• PPP metabolites binding protein determination
• PPP metabolites protein interaction analysis
• PPP metabolites binding site analysis

Applications

Our services can support the following applications, including but not limited to:

• PPP metabolites regulate immune cell function through protein post-translational modification pathways.
• The role and specific mechanisms of PPP metabolites in cancer stem cells.

CD BioGlyco can provide targeted metabolomic detection services for metabolites of the pentose phosphate pathway. Our experienced teams of scientists, researchers, and technicians provide fast turnaround, high-quality services at competitive prices for worldwide customers. Our customers have direct access to our staff and prompt feedback on their inquiries. If you are interested in our services, please contact us for more details.

Reference:

1. Cai Y, Zhu ZJ. A High-Throughput Targeted Metabolomics Workflow for the Detection of 200 Polar Metabolites in Central Carbon Metabolism. Methods Mol Biol. 2019; 1859: 263-274.