Functions of Xylooligosaccharide (XOS)

XOS is a polymer containing 2-7 xylose monomeric units linked by β-(1,4) linkage, which is produced from lignocellulosic materials by chemical or enzymatic methods. XOS acts as a prebiotic, selectively feeding the beneficial bacteria in the digestive tract. XOS has undergone numerous clinical trials demonstrating multiple health benefits, including improvements in blood sugar and blood lipids, improved digestive health, laxatives, and beneficial changes in immune markers. As non-digestible food ingredients for humans, XOS exhibits many bioactivities such as stimulation of the growth of probiotic Bifidogenic and lactic acid bacteriain the gut, improvement of calcium biological availability, reduction of the risk of colon cancer, immunomodulatory properties, antioxidant effects, and antimicrobial activities.

Fig.1 XOS beneficial characteristics as prebiotic in humans. (Valladares-Diestra, et al., 2023)

XOS Analysis Service at CD BioGlyco

Empowered by leading technology and years of experience in biomedical science, CD BioGlyco offers Oligosaccharide Analysis Services for our global clients. As a service provider, our goal is to provide the highest level of service, confidentiality, and support.

• High-performance liquid chromatography (HPLC) analysis

HPLC is considered to be the most powerful technique in analytical chemistry for oligosaccharides because of its efficiency in the separation, identification, and quantification at very low concentrations of any oligosaccharide or compound dissolved in any solvent.

• HPLC with fluorescence detector (HPLC-FLD) analysis

The HPLC-FLD method is applied to products containing large amounts of XOS.

• Gas chromatography coupled to mass spectrometry (GC-MS) analysis

GC-MS is used for the analysis and separation of oligosaccharides.

• High-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) analysis

HPAEC-PAD is used for oligosaccharides identification and determination of complex carbohydrate composition.

• Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) analysis

MALDI-TOF-MS has been known as a fast and responsive technology for acquiring fingerprints of polysaccharides.

Our XOS structural analysis includes:

• Sample preparation
• Sample dilution and filtration
• HPAEC-MS analysis
• Data analysis
• Project report

Fig.2 Structural analysis of XOS by HPAEC-MS. (CD BioGlyco)

Applications

• The structural identification of XOS can provide the basic information for functional food ingredients.
• XOS analysis has been developed for the quality evaluation of functional foods.
• A fast saccharide mapping method is developed based on MADLI-TOF-MS and would be potentially useful to the quality control of XOS products, which may provide some help for the factories producing functional food.
• Xylans under different hydrolysis methods are compared by the HILIC-ELSD method, meanwhile, it is used to monitor XOS contents from different agricultural wastes.

Advantages

• Rapidity, sensitivity, high resolution, and simplicity of the experiments.
• Our dedicated team provides you with innovative, practical, and efficient solutions.
• We provide customized solutions through a deep understanding of needs.

At CD BioGlyco, our experts are dedicated to developing our most advanced products and technologies to help you focus on cutting-edge biomedical research. With the help of our well-established technology and experienced scientists, we provide XOS analysis service for all research and industrial clients. For more information, please feel free to contact us.

Reference:

1. Valladares-Diestra, K.K.; et al. The potential of xylooligosaccharides as prebiotics and their sustainable production from agro-industrial by-products. Foods. 2023, 12(14): 2681.