Gene editing is to make heritable changes by inserting, replacing, deleting, and other targeted modifications or changes of the gene sequence in the gene fragments of organisms. It changes the composition and structure of genes in a directional way, which has the advantages of efficient, controllable, and directional operation.
Current gene editing technologies include homing endonucleases (HEs), zinc finger nuclease (ZFNs), transcription activator-like effector nuclease (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPR), etc. They use nucleases to specifically cut deoxyribonucleic acid (DNA) to form double-stranded breaks (DSB) and carry out the purpose of repairing, to achieve the purpose of modifying the gene fragment. These technologies provide unprecedented convenience and speed for gene editing and have attracted extensive attention from researchers all over the world.
Fig.1 Mechanism of ZFN, TALEN, and CRISPR. (Chen, et al., 2014)
At CD BioGlyco, we have established a best-in-class Glycogenomic Platform, providing clients with services of Glycogene Editing, Glycogene Delivery, Glycogene Expression Profiling, and Cancer Glycogene Discovery Service. A major challenge in the field of glycobiology is to convert complex glycan structures and biosynthetic pathways into more predictable and controllable networks of glycogenes, glycoenzymes, and glycostructures. Nuclease-based precise genome editing technology genetically dissects the glycosylation pathways and biological functions of complex carbohydrates, triggering a revolution in the field of glycobiology.
Fig.2 Basic flowchart of CRISPR-Cas9-mediated genome modification. (CD BioGlyco)
Our technologies for glycogene editing include HEs, ZFNs, TALENs, CRISPR, base editing, and prime editing. With these technologies, we quickly and accurately edit the genome of model organisms, such as pigs that knock out α1,3-galactosyltransferase to eliminate xenotransplantation barriers and edit endogenous genes in almost any cell line. In addition, we also use gene editing technology to explore the role of glycosylation genes and dissect specific structure-function relationships.
CD BioGlyco is committed to providing clients with the highest quality and most comprehensive glycogenomics services. In recent years, we have helped many clients from enterprises, universities, and research institutes solve their difficulties and problems in gene editing. If you are interested in our services, please feel free to contact us for more information.