Natural Oligosaccharide Library Construction Service

Natural Oligosaccharide Library Construction Service

What We Do for Natural Oligosaccharide Library Construction

At CD BioGlyco, our comprehensive Glycan Display Platform is very complete and contains a variety of assay services such as Traditional Glycan Display Arrays, Cell-based Glycan Display Arrays, and Glycophage Display Assays. Oligosaccharide library construction is essential for the preparation of glycan displays. We provide a one-stop natural oligosaccharide library construction service to meet the needs of our clients for traditional glycan display arrays and further apply them in the glycan interaction analysis.

Fig.1 Process of natural oligosaccharide library construction. (CD BioGlyco)Fig.1 Process of natural oligosaccharide library construction. (CD BioGlyco)

  • Process of natural oligosaccharide library construction at CD BioGlyco
    We isolate and characterize naturally occurring oligosaccharides from different sources as a way to construct natural oligosaccharide libraries. The process of construction is as follows.
    • Source Selection
      We select the source from which you want to isolate the oligosaccharides. Natural oligosaccharides are derived from a variety of biological materials, including plants, microorganisms, animals, algae, etc.
    • Material Pre-treatment
      Pre-treatment is performed to facilitate the release of the glycan. Biological materials (cells, tissues, etc.) are impregnated, ground, or homogenized.
    • Extraction of Oligosaccharides
      We extract the oligosaccharides using appropriate solvents. We use different solvents such as water, salt solutions, etc. for extraction. The choice of solvent depends on the solubility and stability of the target oligosaccharide. This process is combined with enzymatic hydrolysis as a way to obtain structurally diverse oligosaccharides.
    • Separation and Purification
      We separate and purify the extracted oligosaccharides by filtration, size exclusion chromatography (SEC), and other methods.
    • Analysis and Characterization
      The structure, composition, and modification of the isolated oligosaccharides are characterized by liquid chromatography (LC), mass spectrometry (MS), nuclear magnetic resonance (NMR), and other methods.
  • Techniques used in the construction of natural oligosaccharide libraries at CD BioGlyco
    • SEC
    • LC
    • MS
    • NMR
    • Other techniques according to your needs

Publication Data

Technology: DNA sequencer-assisted saccharide analysis in high throughput (DASH)

Journal: Biotechnology for Biofuels

IF: 1.522

Published: 2019

Results: In this study, a Miscanthus sinensis grass glucuronoarabinoxylan (GAX) stem and leaf oligosaccharides reference library was constructed by hydrolyzing endo-β-xylanases GH10 and GH11. The library is expressed in glucose units. Analysis of the GAX structures of several species using this library shows that three main GAX sugar side chains are used to decorate xylan molecules. This library provides a very efficient method for xylan structure studies.

Fig.2 Overview of the technical procedure used to characterize GAX structures. (Tryfona, et al., 2019)Fig.2 Overview of the technical procedure used to characterize GAX structures. (Tryfona, et al., 2019)

Applications of Natural Oligosaccharide Library Construction

  • Constructed natural oligosaccharide libraries provide a valuable resource for researchers studying glycan-related biological processes, and glycan-binding proteins.
  • Natural oligosaccharide libraries are used to identify structure-activity relationships, discover novel glycan-protein interactions, and develop glycan-based therapeutics.
  • Natural oligosaccharide libraries are used for rapid screening of functional oligosaccharides or quantitatively binding proteins to affinity glycans.

Advantages of US

  • We offer customization of natural oligosaccharide libraries as per client requirements.
  • The extraction process of oligosaccharides may vary depending on the specific source, material, and research requirements. Depending on the nature of the oligosaccharides required by the client and the limitations of the sample source, we optimize and adjust the extraction protocol.
  • We analyze and characterize the extracted oligosaccharides by a variety of techniques such as NMR, LC, and MS.

CD BioGlyco supports glycan display array-based analysis by constructing structurally diverse natural oligosaccharide libraries. Our rich experience in glycan library construction and advanced instrumentations provide a good guarantee for the natural oligosaccharide library construction service. Please feel free to contact us for more detailed information about natural oligosaccharide extraction and structural characterization.


  1. Tryfona, T.; et al. Development of an oligosaccharide library to characterise the structural variation in glucuronoarabinoxylan in the cell walls of vegetative tissues in grasses. Biotechnology for Biofuels. 2019, 12(1): 1-19.
This service is for Research Use Only, not intended for any clinical use.

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