Cellulose-Hemicellulose-Lignin Content Analysis
CD BioGlyco has advanced technology and equipment and well-trained researchers to provide customers with systematic and rigorous scientific research services. Our rich experience in Cellulose-Hemicellulose-Lignin Content Analysis enables us to provide services more efficiently. We have the confidence to be your essential research assistant in the field of glycobiology.
Cellulose, hemicellulose and lignin are the three major components of plant cell walls. Cellulose is a macromolecular polysaccharide composed of glucose and the most widely distributed and most abundant polysaccharide in nature. and most abundant polysaccharide in nature. Hemicellulose is a heterogeneous polymer composed of several different types of monosaccharides. Its hydrophilic property makes the cell wall swelling and gives fibers elasticity. property makes the cell wall swelling, and give fibers elasticity. Lignin is an amorphous polymer composed of phenylpropane units connected by carbon-carbon bonds and ether bonds. As a typical biomass material, lignin is one of the rare renewable resources among aromatic compounds.
Our investigative protocol is constructed upon a framework of classically validated techniques, integrated with contemporary, high-throughput instrumentation to guarantee both precision and expediency.
This constitutes a principal methodology for the quantitative assessment of cellulose and hemicellulose. The procedure entails employing concentrated acids to depolymerize these intricate polysaccharides into their constituent monosaccharide units, permitting subsequent quantification. Implementation of a sequential sulfuric acid hydrolysis enables effective discrimination and measurement of monosaccharides originating from hemicellulose versus cellulose. This technique represents a rigorous and universally acknowledged benchmark for compositional appraisal.
Post-hydrolysis, the liberated monosaccharides are resolved and quantified via high-performance liquid chromatography (HPLC) coupled with refractive index detection or mass spectrometric (MS) analysis. For lignin quantification, acid-insoluble and acid-soluble fractions are ascertained using UV-Vis spectrophotometry. We further employ an advanced "bottom-up glycomics" strategy that, through chemical depolymerization and MS, facilitates multiplexed, high-throughput, and quantitative dissection of diverse polysaccharides, including cellulose.
Our facility provides an exhaustive analytical provision, meticulously adaptable to a diverse spectrum of biomass feedstocks. We deliver a functionally scalable service architecture—spanning modest investigative endeavors to extensive industrial feedstock characterization—capable of accommodating both raw and pre-processed sample matrices.
We begin by preparing your biomass sample. This involves grinding the material to a uniform particle size and then extracting non-structural compounds such as lipids, proteins, and sugars. This critical step eliminates interference and ensures the analysis focuses solely on the structural components.
The pre-treated sample is subjected to a two-step acid hydrolysis. The first step uses concentrated sulfuric acid to hydrolyze hemicellulose, while the second step uses diluted acid and heat to break down the more resistant cellulose.
The hydrolyzed mixture is filtered to separate the soluble monosaccharides from the insoluble lignin (Klason lignin). We then measure the weight of the residual lignin to determine its content. Acid-soluble lignin is also quantified using UV-Vis spectrophotometry.
The filtrate, containing the sugar monomers from cellulose and hemicellulose, is neutralized and then analyzed by HPLC to determine the concentration of each sugar, such as glucose and xylose.
Our team provides a comprehensive report with the precise weight percentages of cellulose, hemicellulose, and lignin, along with a breakdown of the individual sugars, empowering you to make informed decisions for your project.
DOI.: 10.3390/pr8091048
Journal: Processes
IF: 2.8
Published: 2020
Results: Researchers developed a rapid thermogravimetric method (TGA-PKM) to quantify hemicellulose, cellulose, and lignin in diverse biomass types (e.g., wheat straw, pine bark, poplar). This approach replaces slow, costly chemical analyses by applying deconvolution techniques to derivative thermogravimetric (DTG) pyrolysis curves using a pseudocomponent kinetic model. The method accurately decomposes DTG profiles into contributions from each lignocellulosic fraction based on distinct thermal degradation behaviors: hemicellulose (200–300°C), cellulose (250–380°C), and lignin (200–1000°C). Validation against standard chemical methods showed strong agreement (±7%), confirming its reliability for efficient biomass characterization without complex extraction procedures. The kinetic parameters obtained also provide insights into degradation ease across biomass varieties.
Our cellulose/hemicellulose/lignin content analysis service provides fundamental characterization of terrestrial plant biomass components. Building on this analytical expertise in plant polymers, we extend our services to marine-derived polysaccharides through our Marine Plant Polysaccharide Characterization Service, which includes alginate profiling, fucoidan assessment, and laminarin evaluation for comprehensive marine biomolecule investigation.
CD BioGlyco has provided reliable and cost-effective research services to customers all over the world and has received unanimous praise. We will always adhere to the principle of customer-centricity, keep full transparency in the experimental process and speed up the research of glycobiology for customers.
Customers can contact our employees directly and we will respond promptly. If you are interested in our services, please contact us for more detailed information.
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