HMO Profiling

CD BioGlyco establish advanced technology platforms and introduced numerous professional researchers to ensure that we can provide customers with high-quality, high-precision HMO separation and profiling services. We have the confidence to be your essential research assistant in the field of glycobiology.


For newborns and growing babies, human milk oligosaccharides (HMOs) are regarded as the ideal nutrition source. A better design of new therapeutic agents with anti-infective qualities and better artificial nutrition products will depend on a better knowledge of the features of HMOs, the connection between their structure and function, and the role of HMOs in infant growth and development.

Structural diversity of human milk oligosaccharidesFigure 1. Structural diversity of human milk oligosaccharides (Porfirio, S.; et al. 2020)

As a form of mixed oligosaccharide, HMOs present a challenging separation and quantitative detection task due to the intricacy of their native structure and distinctive chemical characteristics. The sensitivity, repeatability, high output, and speed of the technology and tools are also important factors in the profiling of HMOs. HMOs have been characterized and quantified using a range of chemical methods over the years, including MS, NMR, HPLC, CE, etc.

Representative MALDI-TOF-MS spectrum of permethylated human milk oligosaccharides Figure 2. Representative MALDI-TOF-MS spectrum of permethylated HMOs. (Porfirio, S.; et al. 2020)

HMO Profiling Services at CD BioGlyco

  • HMO Profiling by Methods

There are various techniques for analyzing HMOs. One of the first discovery techniques for oligosaccharide measurement is direct HPLC-UV. Underivatized oligosaccharides and the relative isolation of various isomers can be examined using high-performance anion exchange chromatography (HPAEC) with pulsed amperometric detection (PAD). HMOs can be analyzed using hydrophilic interaction chromatography (HILIC) after being labeled with fluorescence molecules. It is frequently used in conjunction with chromatography because electrospray ionization mass spectrometry (ESI-MS) has a broad measurement range and can provide information about the linking sequence and linkage structure of oligosaccharides. The structure of the glycosidic bonds, the number of residues, the connection, and the linkage pattern between the saccharide units of sugar compounds can all be resolved using NMR.

  • HMO Profiling by Substances

HMO family has many members and they can be determined separately.

HMO are complex unconjugated glycans associated with positive infant health outcomes. HMO profiling is very meaningful, with the function of studying the potential applications of HMO.

Due to the multiple connection possibilities of the monosaccharides used as building blocks, HMOs exhibit very complex structural diversity. Modern analytical technologies are highly required to clarify their structure-function link.

Advantages of Us

  • Combination of multiple technologies
  • Continuously upgrading the technology platform
  • Tailor the experimental program according to the customer's needs
  • Experienced researchers and well-trained technicians
  • Cost-effective

CD BioGlyco provides several high-precision, one-stop, cost-effective HMO profiling services for customers. Our years of experience and expertise have won us praise from customers all over the world. Customers can contact our employees directly and we will respond promptly. Please feel free to contact us for more detailed information if you are interested in our services.


  1. Porfirio, S.; et al. New strategies for profiling and characterization of human milk oligosaccharides. Glycobiology. 2020.
  2. Mantovani, V.; et al. Recent advances on separation and characterization of human milk oligosaccharides. Electrophoresis. 2016, 37(11): 1514-1524.
This service is for Research Use Only, not intended for any clinical use.

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