High-Throughput Automated Micro-Permethylation for Glycan Structure Analysis

High-Throughput Automated Micro-Permethylation for Glycan Structure Analysis

The Importance of High-Throughput Automated Micro-Permethylation for the Efficiency of Glycan Analysis

Traditional methods for glycan analysis require a lot of manual operations and analysis, which are inefficient and error-prone. High-throughput automated micro-permethylation technology allows automatic micro-permethylation operations after certain sample pretreatment. The results are analyzed and measured by high-throughput technical means, substantially improving the efficiency and accuracy of the analysis. In this process, the glycan sample first needs to be pretreated to make it more susceptible to the action of micro-permethylation reagents. Next, the micro-permethylation reaction is performed automatically using high-throughput automated equipment to methylate the glycan molecules therein. Finally, the samples are analyzed and detected by mass spectrometry to derive information about the glycan structure. This technique is an important advance in glycan structure analysis as it effectively reduces the time and errors of manual manipulation. By using this technique, researchers can rapidly and accurately analyze a large number of glycan structures and decipher their functions and roles.

Schematic diagram of the permethylation procedure using an automated 96-well pipet.Fig.1 Schematic diagram of the permethylation procedure using an automated 96-well pipet. (Shajahan, et al., 2018)

High-throughput Automated Micro-permethylation for GlycanStructure Analysis at CD BioGlyco

CD BioGlyco is committed to providing comprehensive solutions to our clients through advanced technologies and high-quality services. We provide services related to analyzing glycan structures by high-throughput automated micro-permethylation. Our services enable more precise analysis and research on different glycan structures. The specific services we offer include but are not limited to the listed below:

  • We combined micro-permethylation with electrospray ionization mass spectrometry (EMI-MS) for the analysis of glycan structures. After the micro-permethylation of glycan molecules, the structures are analyzed in a high-throughput way using EMI-MS. This service produces accurate glycan analysis results in a short period and presents the results in a visualized form that is accessible for analysis and understanding.
  • The micro-permethylation is combined with matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) to analyze the structure of the glycans. Similarly, after micro-permethylation of the sugar molecule, we choose the appropriate mass spectrometry, MALDI-MS or Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS), depending on the molecular weight of the glycans, to perform the high-throughput analysis of their structures.

The process of glycan structure analysis by high-throughput automated micro-permethylation.Fig.2 The process of glycan structure analysis by high-throughput automated micro-permethylation. (CD BioGlyco)


  • Synthesis of more complex organic substances or drugs
  • Research on intracellular molecular recognition
  • Research related to the preparation of vaccines


  • Efficient data analysis and evaluation reports
  • Outstanding client support in the field of glycochemistry
  • High-quality technologies and methods

CD BioGlyco offers the service of analyzing glycan structures by high-throughput automated micro-permethylation technology. Our service helps our clients analyze complex glycan structures quickly and accurately to advance their relevant research. If you are interested in our services, please feel free to contact us for further information.


  1. Shajahan, A.; et al. High-throughput automated micro-permethylation for glycan structure analysis. Analytical chemistry. 2018, 91(2): 1237-1240.
This service is for Research Use Only, not intended for any clinical use.

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