Sugar-nucleotides are of great significance in mediating biological processes and the development of carbohydrate drugs. CD BioGlyco has many years of experience in sugar-nucleotides synthesis, and can customize high-quality synthesis services according to customers' research needs. We have confidence to be your essential research assistant in the field of glycobiology.

Background

Structurally, sugar-nucleotides are composed of a sugar or sugar derivative and a nucleoside monophosphate or diphosphate. Sugar-nucleotides are necessary intermediates for carbohydrate metabolism and glycoconjugate biosynthesis. The biosynthesis of glycans and glycoconjugates depends on the activation of monosaccharides as sugar-nucleotides. They are composed of an activated sugar donor that is glycosylated onto a diverse range of acceptors, typified by glycosyltransferase catalyzed processes for the assembly of glycosides and oligo- or polysaccharides.

Sugar-nucleotides and structural analogs can be used as substrates for enzymatic reactions in carbohydrate synthesis, as powerful tools for studying glycoconjugate biosynthesis, and they have the potential as enzyme inhibitors in new therapeutic strategies. Besides, they are also important for the provision of structurally defined homogenous analytical standards.

Therefore, the effective preparation of natural and unnatural sugar-nucleotides is of great significance in both synthetic biology and medicinal chemistry. The synthesis of sugar-nucleotides is more difficult because of many factors: Sugar-nucleotides synthesis can be difficult due to many factors: the low solubility of sugar nucleotides in organic solvents, the presence of polar or charged functional groups, as well as the sensitivity of glycosidic and pyrophosphate bonds to hydrolytic cleavage.

Fig 1. Representative examples of common mammalian sugar-nucleotides (Ahmadipour, S.; et al.)

Services

CD BioGlyco provides a variety of professional sugar-nucleotides synthesis services. Consumers have the option to choose what suits their research plan the most.

• Chemical synthesis can prepare various natural and unnatural structures. This method has multiple steps and is challenging.
• Enzymatic synthesis has the characteristics of high location specificity and stereospecificity. It simulates the synthesis steps of sugar chains in organisms and is an effective alternative to organic chemical synthesis.
• Combining chemical and enzymatic methods. Firstly, the core precursor is prepared by chemical synthesis, and then the precursor is expanded into a variety of complex glycans by glycosyltransferase catalysis.

Applications

• Development of tools to study the biosynthesis of carbohydrates and glycoconjugates
• Enzyme inhibitor development
• Carbohydrate structure and function research

Advantages of Us

• Able to synthesize a variety of nucleotide sugars
• Outstanding process development, optimization and scale-up
• Customized route design
• Selection of the appropriate counter ion to phosphate group in the molecule to preserve the high purity

CD BioGlyco can customize different experimental programs to meet the different research needs of customs. We have first-class experimental equipment and experienced researchers to provide you with professional sugar-nucleotides synthesis services.

Customers can contact our employees directly and we will respond promptly. If you are interested in our services, please contact us for more detailed information.

References:

1. Wagner, G.K.; et al. A survey of chemical methods for sugar-nucleotide synthesis. Nat. Prod. Rep. 2009, 26: 1172-1194.
2. Ahmadipour, S.; et al. Recent advances in the enzymatic synthesis of sugar-nucleotides using nucleotidylyltransferases and glycosyltransferases. Carbohydrate Research. 2018.