Dextrose Analysis Service

Clarity in Complexity: Dextrose Analysis Made Simple

Dextrose (D-glucose) is a simple sugar that is the primary source of energy for living organisms. Detecting and characterizing it accurately is essential in pharmaceuticals and scientific research. CD BioGlyco is a specialist company in Pharmaceutical and Biological Analysis Services, providing comprehensive dextrose analysis services. Our team of experienced scientists utilizes state-of-the-art technology to determine the presence and purity of dextrose in a variety of samples. We perform a variety of tests including identification, quantitative analysis, impurity testing, and specific tests for solution color and clarity, conductivity, dextrin, soluble starch, and sulfites.

Fig.1 Dextrose analysis service. (CD BioGlyco)Fig.1 Dextrose analysis service. (CD BioGlyco)

  • Identification
    • Infrared spectroscopy (IR): We determine dextrose's functional groups by analyzing IR, thereby confirming its chemical structure.
    • Hydrogen nuclear magnetic resonance spectroscopy (1H-NMR) and carbon spectrum (13C-NMR): These two modalities provide insights into the chemical environment of hydrogen and carbon atoms within the molecules of dextrose, further verifying their molecular structure.
  • Quantitative analysis
    • Titration: We calculate its quantity by using a reagent of known concentration to chemically react with dextrose.
    • High-performance liquid chromatography (HPLC): We accurately determine the content of dextrose with HPLC by integrating different chromatographic columns and detectors. This method boasts high resolution and sensitivity, making it suitable for the analysis of complex samples.
    • Ultraviolet-visible spectroscopy (UV-Vis): The content can be identified using either the standard curve method or the comparison method by measuring dextrose's absorbance at a specific wavelength. This technique is fast and easy, rendering it ideal for the quick analysis of numerous samples.
  • Impurity tests

We identify impurities in glucose using analytical methods such as thin-layer chromatography (TLC) and gas chromatography-mass spectrometry (GC-MS). These techniques are efficient in identifying any impurities that might coexist with dextrose in a sample.

  • Specific tests
    • Color and clarity of solution: We examine the color and transparency of the dextrose solution, comparing it to predetermined standards to ascertain if the color and clarity meet the required criteria.
    • Conductivity: We measure the conductivity of the dextrose solution to initially determine whether there are ionic impurities in it. Conductivity values should fall within a specific range to meet standard requirements. This test helps to quickly determine the ion content of a sample to assess glucose purity.
    • Dextrin: Dextrin is a by-product formed during the hydrolysis of dextrose. We perform purity assessments by quantifying dextrins in glucose samples using specific test methods such as titration or chromatography. This testing is critical to control product quality and ensure consistency of purity from batch to batch.
    • Soluble starch: Soluble starch is the polymerization product of glucose. We use specific tests to evaluate whether the content in glucose samples meets standard requirements. Such testing helps ensure product consistency and purity.
    • Sulfites: Sulfites are common impurities that can be found in dextrose samples. We use specific tests such as titration or chromatography to detect the sulfite content in glucose samples.

Importantly, we also provide analyses of 2 injections: dextrose and sodium chloride injection, and dextrose injection.

  • For the analysis of dextrose and sodium chloride injection, the following analysis is added in addition to all dextrose analysis items: sodium chloride content, pH, limit of 5-hvdroxymethylfurfural and related substances, and bacterial endotoxins test.
  • For the analysis of dextrose injection, the following analysis is added in addition to all dextrose analysis items: limit of 5-hvdroxvmethvlfurfural and related substances, pH, bacterial endotoxins test, and particulate matter in injections.

Publication Data

Technology: Prolotherapy

Journal: The Korean journal of pain

IF: 2.6

Published: 2021

Results: The authors conducted an investigation into the impact of low concentrations of dextrose and lidocaine on fibroblasts, proposing an optimal concentration range for use in prolotherapy. Utilizing western blot analysis, extracellular signal-regulated kinase (Erk) activation, as well as the protein expression of collagen I and α-smooth muscle actin (α-SMA), were scrutinized. Findings revealed a decline in cell viability at dextrose levels exceeding 5% and lidocaine concentration above 0.1%. The suppression of Erk activation proved to inhibit fibroblast proliferation and collagen I synthesis.

Here are some of the results shown in this article:

Fig.2 Trypan blue exclusion assay after treating the various concentrations of dextrose for 24 hours in NIH-3T3. (Woo, et al., 2021)Fig.2 Trypan blue exclusion assay after treating the various concentrations of dextrose for 24 hours in NIH-3T3. (Woo, et al., 2021)

Frequently Asked Questions

  • What are the functional properties of dextrose?

Dextrose has a good effect of replenishing energy and water due to its easy absorption properties.

  • How precise and accurate is your dextrose analysis service?

We use sophisticated analytical techniques and methodologies to ensure high precision and accuracy in our analysis. Our experienced team follows stringent quality controls and validated methods to provide you with reliable results. The exact precision and accuracy can, however, vary depending on the specific nature of the sample being analyzed. We recommend discussing these specifics with our research team for a more detailed understanding.

As a part of our Pharmaceutical Analysis Services, CD Bioglyco offers a comprehensive dextrose analysis service. This service is geared towards providing detailed characterization and quantification of dextrose and sodium chloride injection, as well as just dextrose injection. We warmly invite interested clients to contact us for further information on how we can support specific needs.


  1. Woo, M.S.; et al. The proper concentrations of dextrose and lidocaine in regenerative injection therapy: in vitro study. The Korean journal of pain. 2021, 34(1): 19-26.
This service is for Research Use Only, not intended for any clinical use.

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CD BioGlyco is a world-class biotechnology company with offices in many countries. Our products and services provide a viable option to what is otherwise available.

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