Polydextrose Analysis Service

Promote Your Project Development with Professional Polydextrose Analysis

Polydextrose is a randomly cross-linked polymer of D-glucose, sorbitol, and citric acid or phosphoric acid by high-temperature melt polycondensation. It is a water-soluble dietary fiber. CD BioGlyco provides high-quality Pharmaceutical Excipient Analysis services such as Sucrose Octaacetate Analysis, Sugar Sphere Analysis, and Sucrose Stearate Analysis. We look forward to being your best partner in Pharmaceutical and Biological Analysis. Moreover, our team provides reliable Pharmaceutical Analysis and Biological Evaluation services.

  • Professioan identification
    • Chromogenic reaction: Our researchers add sulfuric acid quickly after adding phenol solution dropwise to the diluted polydextrose solution, the color is a dark yellow to orange color.
    • Our researchers add a trace amount of acetone to the polydextrose test solution to observe the clarification of the solution. Then, we add acetone to the clarified solution and shook it well to observe the milky white precipitate.
    • Our researchers add alkaline copper citrate solution to the diluted polydextrose test solution, boil it, and observe the color of the supernatant. The color is blue or blue-green.
  • Molecular weight determination
    • We use size exclusion chromatography (SEC) to determine the molecular weight of polydextrose. Our researchers optimized the detector, chromatographic separation conditions, and buffer to ensure the best separation.
  • Quantitative analysis
    • 5-Hydroxymethylfurfural: Our team provides high-quality 5-hydroxymethylfurfural quantification service by ultraviolet-visible (UV-Vis) spectrophotometry with absorbance at 284 nm.
    • Glucose, sorbitol, and 1,6-anhydro-D-glucose: Our researchers dissolve glucose, sorbitol, and 1,6-anhydro-D-glucose standard as a control. We determine the control and polydextrose test solutions using high-performance liquid chromatography (HPLC). Our researchers calculate the amount of glucose, sorbitol, and 1,6-anhydro-D-glucose in the polydextrose test solution separately according to the external standard method.
    • Polydextrose: We use cation exchange chromatography to determine polydextrose test solutions and polydextrose standard solutions, respectively. Our researchers analyze the content by external standard methods.
  • Basic analysis
    • Our team provides some quick tests such as pH tests, moisture content tests, scorch residue tests, and heavy metal test services.

Schematic diagram of polydextrose analysis. (CD BioGlyco)

Publication Data

Technology: Metabolomic analysis, Non-targeted ultra-high-performance liquid chromatography-quadrupole time of flight mass spectrometry (UHPLC-qTOF-MS) metabolite profiling analysis, Reversed-phase (RP) and hydrophilic interaction (HILIC) chromatography

Journal: Scientific Reports

Published: 2020

IF: 4.6

Results: In this work, the researchers used a non-metabolomic approach to analyze the metabolism of blood and epididymal adipose tissue samples from mice with oral administration of polydextrose (PDX mice). Metabolomics analysis showed highly significant changes in PDX mice compared to control mice. Analysis of the cecum microbiota indicated that the bacterial genus in PDX mice was associated with a benefit in metabolic effects related to lipid metabolism.

Fig.1 Metabolite pathway analysis.Fig.1 Pathway mapping with metabolites. (Saarinen, et al., 2020)

Applications of Polydextrose Analysis

  • Polydextrose analysis plays a key role in the metabolomic analysis of animal polydextrose models.
  • Comprehensive polydextrose analysis further facilitates the development of dietary fiber formulations.
  • Polydextrose analysis expands the development and utilization of polydextrose in flavorings, dairy products, and wine.

CD BioGlyco has a strict control system and skillful operators to provide polydextrose analysis to our clients. We have a knowledgeable operational team to help clients plan and complete projects. We encourage you to contact us if you have any concerns.

Reference

  1. Saarinen, M.T.; et al. Metabolomics analysis of plasma and adipose tissue samples from mice orally administered with polydextrose and correlations with cecal microbiota. Scientific Reports. 2020, 10: 21577.
This service is for Research Use Only, not intended for any clinical use.

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