The method of lectin enrichment is widely used in N-linked glycopeptide enrichment. CD BioGlyco is committed to improving the identification ability of glycosylation sites and the enrichment efficiency of glycopeptides, to achieve the goal of providing efficient services to customers. We are confident to be your indispensable research assistant in the field of glycobiology.
Glycosylation is one of the most important post-translational modifications of proteins and is ubiquitous in organisms. Large-scale N-linked glycosylation site identification is an important part of glycoproteomics research. Therefore the enrichment of N-linked glycopeptide is crucial for the high-throughput identification of N-glycosylation sites. Due to the high heterogeneity of glycosylation sites and modifications, the complexity of glycosylated peptide detection is increased. Glycoproteins or glycopeptides should be enriched to improve the detection sensitivity.
Lectins have the property of recognizing carbohydrates through carbohydrate binding sites. The lectin enrichment method is to selectively identify some monosaccharides or glycans with special structures by lectins to realize the enrichment of glycoproteins. Different types of glycopeptides can be enriched by different lectins or a combination of various lectins. For example, it was reported that the N-linked glycosylation sites of the brain, kidney, liver, and heart of mice were comprehensively interpreted by using three lectins (convalina agglutinin, wheat germ agglutinin, and ricinus communis agglutinin I).
Fig.1 Sample preparation and enrichment of N-glycosylated peptides. (Zielinska, 2010)
CD BioGlyco has established a variety of lectin-based enrichment strategies for improved coverage of glycan analysis. The methods we provide include but are not limited to:
At CD BioGlyco, our researchers constantly study cutting-edge knowledge in the field of glycobiology to actively improve our experimental process and help our customers achieve their goals in a timely, efficient, and economical manner. If you are interested in our services, please contact us and we will respond immediately and try our best to offer scientific assistance.