CD BioGlyco is a world-leading company that can provide the best solutions for global customers in the field of microarrays. We have successfully completed many scientific research and non-clinical research projects to ensure that we provide the best products and services to customers all over the world.

Microbial Glycan Research and Microarray

Studying the natural host ligands of microorganisms and understanding their pathogenicity and infectivity has become one of the goals of functional glycomics. The interaction between glycan-binding protein (GBP) and its carbohydrate ligand is the basis of all aspects of life. In order to better understand proteoglycan recognition, the specificity of many structurally different glycans must be determined. Glycan arrays are excellent tools for glycan-binding analysis because they use a limited amount of samples to quickly interrogate binding events. Chemically diverse microbial glycans play a vital role in immunity and infectious diseases. Since microorganisms are wrapped in complex carbohydrates, better coverage is needed. Current microbial glycan arrays contain specialized small synthetic or isolated carbohydrates.

Fig. 1 Bacterial glycans and cell wall structures. (Campanero-Rhodes, et al., 2020)

Service Process

CD BioGlyco provides microbial glycan group printing microarrays as a tool for studying microbial glycobiology. The collection of synthetic glycans used in our microarray service covers the surface of pathogens and other microorganisms as carbohydrate structures for intrusion recognition, recognition by immunoagglutinins and receptors, and recognition by interaction with bacterial lectins. Arrays resulting from the surface attachment of well-defined synthetic glycans can elucidate binding events with atomic resolution. Arrays formed by isolated compounds can be used for glycomics research on target microorganisms.

• The printed glycan array helps to clarify the role of glycans in many biological processes with monosaccharide resolution, especially those of mammalian origin.
• Glycan arrays and other array platforms have been used to study the interaction of glycans with microorganisms and important microbial components (such as toxins, adhesins, and lectins) in various attachment and toxicity pathways.

Fig 2. Flow chart of microbial focused glycan array screening service of CD BioGlyco.

Workflow

• Microarray printing: Our free glycan library features a diverse range of carefully synthesized or isolated glycan structures. These glycans are fixed to a solid substrate at high density.
• Sample incubation: Our service is applicable for recognition analysis between different hosts, phages, glycoproteins (e.g., lectins, immune system effectors, bacterial and phage cell wall lysozymes), and microorganisms. After the sample is labeled, it is incubated with the microarray under strictly controlled physiological conditions (temperature, buffer composition, incubation time) to promote specific binding. Subsequently, optimized washing steps are performed to remove any non-specific binding substances.
• High-sensitivity detection: After incubation and rigorous washing steps to remove non-specific binding substances, we use a high-resolution microarray scanner for scanning.
• Binding profile analysis: We conduct a detailed analysis of the observed binding profiles and convert them into clear, actionable insights for research applications such as potential pathogenic mechanisms and host specificity.

Applications

• Our microarrays are suitable for analyzing interactions between different hosts and microbial pathogenic factors, providing a basis for rational drug design.
• Our microarrays are suitable for detecting antibodies against multiple bacteria in serum, playing a significant role in serological diagnosis research for bacterial infections.
• Our microarrays are used to assist in identifying bacterial structures that induce immune responses and analyzing the specificity of epitopes recognized by protective antibodies induced by vaccines, thereby accelerating the development of effective vaccines to prevent bacterial infections.

Our Features and Benefits

• The primary advantage of using microarrays lies in their inherent miniaturization, which enables sensitive and high-throughput analysis using minimal sample quantities.
• Our microarrays cover a wide range of glycan structures, providing reliable analytical tools for understanding specific recognition events.
• Our microarray designs are compatible with a broad spectrum of microbial species, offering extensive application value across multiple fields of microbiology, from infectious diseases to environmental microbiology.

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Frequently Asked Questions

CD BioGlyco provides microbial focused glycan array screening and analysis services. We have a well-equipped experimental platform, a large and professional team of scientists, and we are committed to cooperating with researchers from all over the world to meet customer needs. Customers can contact our employees directly and provide timely feedback on their queries. If you are interested in our one-stop solution service, please contact us for more detailed information.

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