There are considerable differences between bacterial and mammalian glycans. In contrast to the most eukaryote, bacterial glycans are usually composed of a large number of repeating units. The bacteria cell wall is based on peptidoglycan network macromolecular structure, that is, a polypeptide chain, in which polysaccharide chains are cross-linked with short peptide chains. Gram-negative bacteria have an outer wall composed of lipopolysaccharide-protein complexes. Lipopolysaccharide contains lipid A, the phosphorylated GlcN-GlcN disaccharide, and N- and O-acylated with fatty acids that anchor the molecule in the leaflet of the outer membrane.
Since these repeating units are usually displayed on the cell surface, they can interact with the environment. These carbohydrates also contain antigenic determinants, can elicit an immunogenic response in the host, and can provide identification elements for pathogens such as phages. We provide services on bacterial surface carbohydrates to help expand the general knowledge and understanding of the complexity of glycans in biological systems, which will provide a basis to design high-throughput vaccines, develop diagnostics and immunostimulants.
Fig 1. Cell wall structure of Gram-positive and Gram-negative bacteria (Kang, S.S.; et al. 2016)
We provide highly selective peptidoglycan purification services before detailed structural characterization, which can lay a good foundation for customers' subsequent experiments.
We provide highly selective Lipopolysaccharide purification services before detailed structural characterization, which can lay a good foundation for customers' subsequent experiments.
CD BioGlyco uses kinds of technology platforms and methods, under the operation of well-trained researchers, to provide customers with the highest quality and most reliable bacterial carbohydrates analysis services. We hope to start the exploration of glycobiology with you.
Customers can contact our employees directly and we will respond promptly. If you are interested in our services, please contact us for more detailed information.