CD BioGlyco has developed chemical synthesis methods and enzyme synthesis methods to provide our customers with high-quality customized sialyl Lewis x antigen and its derivatives production services. We are confident to be your scientific assistant in the field of glycobiology.

What is Sialyl Lewis x Antigen?

Typical tumor-associated carbohydrate antigens are two carbohydrate structures named sialyl Lewis a and sialyl Lewis x. Sialyl Lewis x, a member of body carbohydrate, is an inherent blood-type tetrasaccharide on the surface of different cells, the lymphocyte, neutrophil, some T cells, multiple tumor cells and so on. Sialyl Lewis x is the most important ligand of the three selectins, L-selectin, E-selectin, and P-selectin, and plays important role in multiple physiological phenomena by interacting with selectins. For example, the expression of sialyl Lewis x on leukocytes contributes to their function in the inflammatory response via interaction with E-selectin expressed on endothelial cells. Sialyl Lewis x is involved in the recruitment of leukocytes to lymphoid tissues and inflammation sites.

The biosynthesis of sialylated Lewis antigens (sialyl Lewis a and sialyl Lewis x) requires first the α2,3-sialylation of Gal prior toα-1,3/4-fucosylation. ST3Gal IV and ST3Gal VI mainly act on type 2 disaccharides, leading to the biosynthesis of sialyl Lewis x. For the subsequent fucosylation, FucT VII shows a restricted substrate-specific city since it forms only sialyl Lewis x.

Fig.1 Structure of sialyl Lewis x antigen. (Wikipedia)

Our Services

CD BioGlyco offers high-quality sialyl Lewis a synthesis and analysis service according to customers' research needs. Our services include but are not limited to:

• Chemical synthesis of sialyl Lewis x and its derivatives. For example, we synthesize several sialyl Lewis x glycoconjugates including sialyl dimeric Lewis x, fluorescent sialyl Lewis x glycosphingolipids, sialyl trimeric Lewis x, sialyl Lewis x glycosphingolipids. And these compounds will be coupled with protein and then be used to conduct further preclinical studies for the diagnosis of cancer.
• We also provide sialyl Lewis x synthesis by enzymatic synthesis strategy, which employs enzyme nucleotide donor recycling. Glycosyltransferases have become valuable tools for regio- and stereoselective glycosylations. Sialyl Lewis x is prepared by using β1,4-galactosyltransferase (GalT) to form Galβ1,4GlcNAc, recombinant α-2,3-sialyltransferase (SiaT) to form NeuAcα2,3Galβ1,4GlcNAc, and α-1,3-fucosyltransferase(FucT) to form NeuAcα2,3Galβ1,4(Fucα1,3)GlcNAc. This three-enzyme system has been utilized extensively later on in the synthesis of sialyl Lewis x.
• Chemoenzymatic synthesis of O-sulfated sialyl Lewis x antigens containing different sialic acid forms and O-sulfation at different locations using one-pot multienzyme (OPME) sialylation systems. O-Sulfated sialyl Lewis x structures play important roles in immune regulation, inflammation, and cancer metastasis.
• Structural analysis of sialyl Lewis x using 2D NMR

Advantages of Us

• Optimized synthesis service of sialyl Lewis x and its derivatives
• Customized solutions
• A combination of multiple synthesis techniques
• Efficient and reliable

CD BioGlyco is one of the most reliable partners equipped with experienced scientists. We are committed to providing you with reliable products of sialyl Lewis x antigen. If you are interested in our services, please contact us for more details.

References

1. Ugorski, M.; Laskowska, A. Sialyl Lewis (a): a tumor-associated carbohydrate antigen involved in adhesion and metastatic potential of cancer cells. Acta Biochimica Polonica. 2002, 49(2): 303-311.
2. Pudelko, M.; et al. Chemical and chemoenzymatic synthesis of glycopeptide selectin ligands containing sialyl Lewis X structures. ChemBioChem. 2010, 11(7): 904-930.