Excellent Human Rabies Immunoglobulin Evaluation Rely on Professional Team

Human rabies immunoglobulin (HRIG) is made from healthy human plasma containing high-potency rabies antibodies after separation, purification, sterilization, and inactivation. CD BioGlyco has a professional and reliable Pharmaceutical and Biological Analysis team to provide accurate Pharmaceutical Analysis, Biological Evaluation, and Pharmaceutical Excipient Analysis. Moreover, we provide excellent evaluation for Human Hepatitis B Immunoglobulin Evaluation, Human Tetanus Immunoglobulin Evaluation, Human Immunoglobulin Evaluation, and Anti-human T Lymphocyte Rabbit Immunoglobulin Evaluation services. Whatever you need in pharmaceutical and biological, we provide the most satisfactory solution for you.

CD BioGlyco mainly measures three states of HRIG including bulk, final bulk, and final product.

• Basic analysis
  • pH test
  • Surface test
  • Heat stability test

  • Purity test
  • Visible particulate test
  • Abnormal toxicity test

  • Pyrogen test
  • Sterility test
• Content analysis
  CD BioGlyco provides accurate protein, residual ethanol, sugar, and glycine content determination services.
  • Our researchers determine the protein content of the final bulk and final product by biuret and Kjeldahl method respectively.
  • The residual ethanol content is calculated by measuring the absorbance using the dish diffusion method.
  • For sugar and glycine content, we use high-performance liquid chromatography (HPLC) to provide accurate quantification. Notably, glycine reacts with an excess of 6-aminoquinolinyl-N-hydroxysuccinimidylcarbamate (AQC) for pre-column derivatization.
• Molecular size distribution
  • We use size exclusion chromatography (SEC) to determine the IgG monomer and dimer content of HRIG and the separation between the monomer peak and the cleavage peak.
• Final product identification
  • Double immunodiffusion assay: We add HRIG and anti-human, anti-horse, anti-bovine, anti-pig, and anti-sheep serum or plasma to two adjacent wells of an agarose gel plate. After a while, a distinct precipitation line forms only with the anti-human serum or plasma.
  • Immune electrophoresis: We separate HRIG by electrophoresis and observe the position and shape of its precipitation arc.
• Rabies antibody potency
  • For antibody potency, we mainly provide two analysis services including a mouse neutralization test (MNT) and a rapid fluorescent focus inhibition test (RFFIT).
  • For anti-HBs, we provide professional radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA) services.

Publication Data

Applications of Human Rabies Immunoglobulin Evaluation

• HRIG evaluation raises awareness of the disease and develops the next generation of vaccines to effectively prevent infection.
• HRIG evaluation helps to further elucidate the extent and nature of RIG interference with new vaccine candidates.
• HRIG evaluation plays a key role in the development of similar types of immunoglobulin products.

Advantages of Us

• Based on the pharmaceutical and biological analysis needs of our clients, our professional staff plans the analysis and evaluation program.
• Our SEC analysis is also suitable for the analysis of IgG monomer and dimer content in human immunoglobulin analogs.
• The RFFIT we provide is very reproducible and requires less sample volume.

CD BioGlyco provides systematic pharmaceutical and biological analysis services to our clients in the research business and industry worldwide. Our efficient biological evaluation team makes every effort to fulfill our client's every need. Contact us at your convenience for assistance.