Services Related to LiGA-based Glycan Modification Starts with the Details

CD BioGlyco is skilled in all experiments of Glycan Display Arrays including LiGA and others. LiGA-based glycan modification plays an important role in the construction of LiGA and various glycan arrays. We provide a one-stop service for LiGA-based glycan modification. We synthesize structurally diverse glycans by modification, ultimately expanding the applications of LiGA. We mainly modify glycans from the following two aspects.

• Synthesis of glycans with alkyl-azido linker

We link a variety of alkyl azides to glycans, including benzyl azide, n-heptyl azide, cyclohexyl azide, phenylethyl azide, methyl azidoacetate, and others.

• Synthesis of azido-glycan

We synthesize azido-glycan by linking various azides, such as 8-azido-octanol, to glycan. During the synthesis, we always check the synthesis of the glycan conjugates to make sure that the structure is accurate.

Fig.1 Process for the synthesis of glycans with alkyl-azido linkers of azidos synthesis. (CD BioGlyco)

• Process of LiGA-based glycan modification at CD BioGlyco

The experimental flow of our LiGA-based glycan modification consists of the following main parts:

• Confirmation of glycan structure
  We determine the structure of the glycan required for the reaction according to client requirements. Glycans are obtained from natural sources or custom synthesized.
• Protection/deprotection
  We identify the groups on the carbohydrate backbone that need to be protected to ensure selective reactions at specific sites. We use appropriate chemical methods to protect the groups.
• Synthesis of alkyl azide linkers or azides
  We synthesize the required azides and alkyl azides, including methyl azidoacetate, n-heptyl azide, 8-azido-octanol, and others.
• Attachment of glycans to alkyl azides or azides
  We activate glycans and azides or alkyl azides for further functionalization. We link the azide or alkyl azide to the glycan through various coupling chemical reactions. The reactivity and functionality of the glycan are restored by removing the protecting groups from the glycan molecule.
• Purification and characterization
  We are equipped with a variety of state-of-the-art equipment to purify and characterize the obtained modified glycans, including mass spectrometry and nuclear magnetic resonance. After testing the structure and purity, modified glycans are used to construct LiGA.

Our specific experimental protocols and conditions vary depending on the specific glycan structure, alkyl azide, azides, and desired application. During synthesis, we also consider the water solubility, stability, potential reactivity, etc., of the glycan linker. We also validate the availability of these glycans by constructing LiGAs and testing their functionality.

Publication Data

Applications of LiGA-based Glycan Modification

• Modified glycans can be used in the construction of Liquid Glycan Display Arrays to analyze the interaction of glycans with glycan-binding proteins.
• Modified glycans are used to conjugate with a variety of molecules such as lipids and nanoparticles to construct functional tools.
• Modified glycans are used to assay the biological activity and functionality of glycan conjugates in organisms.

Advantages of Us

• We use a variety of techniques to analyze and characterize the structure of modified glycans to confirm successful construction.
• We further optimize the experimental process, modify the glycan, etc., depending on the specific application and desired properties.
• We have the expertise in carbohydrate chemistry and bioconjugation to tackle the synthesis of modified glycans.

CD BioGlyco combines knowledge related to glycobiology and chemistry to solve various problems encountered in glycan analysis. We are fully capable of synthesizing various modified glycans. In addition, we also provide other services, such as LiGA Construction, Quality Control of LiGA, and Functional Validation of LiGA. If you are interested in our glycan modification, please feel free to contact us for information on experimental procedures.