Hydrophilic interaction chromatography (HILIC) is a valuable analytical tool for biochemical research. CD BioGlyco has accumulated extensive experience in the application of HILIC to the analysis of glycosylation. Based on our extensive expertise and advanced research platform, we are confident to provide efficient and reliable technical support to our customers.

Background

Characterization of protein glycosylation has significance for understanding protein function and role in various disease states. Glycosylation sites and the composition, branching, and length of glycan chains can affect the structure and function of proteins. This glycosylation information is important for understanding proteins used as therapeutic agents. Therefore, a variety of separation techniques have been developed to analyze and characterize glycans from glycoproteins, proteoglycans, and glycolipid conjugates.

The polarity of glycopeptides and oligosaccharides interact selectively with the polar stationary phase, which provides an opportunity for the application of HILIC in sample purification and analysis. HILIC has been successfully applied to several aspects of glycosylation analysis. PNGase F was used to perform glycan profile analysis of enzymatic glycan release, followed by derivatization with 2-aminobenzamide (2-AB) by HILIC-UHPLC, which is a powerful technique that can be used for the analysis of samples with well-known N-glycan profiles.

Fig.1 HILIC combines the characteristics of the three major methods in liquid chromatography. (Buszewski, 2012)

Our Services

CD BioGlyco focuses on the development of multiple isolation and purification techniques to characterize glycans from glycoconjugates. Currently, we have established efficient and accurate HILIC analytical strategies and we provide our clients with high-quality preliminary identification services for a wide range of glycans. Services we provide include but are not limited to:

• Fluorescence detection of labeled glycans
• Mass spectrometric analysis after HILIC separation
• Release of glycans from glycoconjugates and labeling for detection
• Profile comparisons to monitor known structures

Workflow for HILIC Profiling

Applications

• Drug composition analysis and drug impurity analysis
• Research on medicine modernization
• Study on drug metabolism and kinetic analysis

Advantages of Us

• We provide feasibility analysis before detection and post-test result analysis report.
• We provide flexible and reliable analysis processes according to customers' project needs
• Our professional research team will continuously follow the project progress and provide real-time guidance.

Equipped with first-class instruments and standardized operating procedures, CD BioGlyco provides efficient services of glycan separation and purification. In addition, we have constructed a database to aid in the interpretation of HILIC glycan profiles. Please feel free to contact us for more details if you are interested in our services.

Reference:

1. Buszewski, B.; Noga, S. Hydrophilic interaction liquid chromatography (HILIC)-a powerful separation technique. Analytical & Bioanalytical Chemistry. 2012, 402(1):231-247.