Sucralose Analysis Service
Sweeten Your Success with Seamless Sucralose Analysis
As a biological industry leader, CD BioGlyco has been working in the field of Pharmaceutical Excipient Analysis for a long time. Here, CD BioGlyco provides professional and high-quality sucralose analysis to clients. Sucralose has the molecular formula C12H19Cl3O8 and appears as a white or off-white crystalline powder. Sucralose is the only functional sweetener made from sucrose, which can be up to 600 times sweeter than sucrose. In addition, sucralose does not participate in metabolism in the body, is not absorbed by the body, and has zero calorific value, so it can be used as an ideal medicinal excipient for diabetics.
- Identification of sucralose
- Chromatography. The chromatogram of the sample to be tested is determined after dissolving it in methanol. Finally, the chromatogram of the sample is compared with the chromatogram of a sucralose standard. If the two spectra agree, the sample is proved to be sucralose.
- Infrared spectroscopy. Similarly, we compare the infrared spectrogram of the sample with the infrared spectrogram of the sucralose standard (compare the intensity and position of the absorption peaks). If the two spectra agree, the sample is proven to be sucralose.
- Specific rotation of sucralose
Sucralose is dissolved well in water and then we test its specific rotation using a spinometer. The specific rotation of sucralose should be between +84.0° and +87.5°. - Sucralose content analysis
An effective method of determining the sucralose content is high performance liquid chromatography (HPLC). We determine the chromatogram using water-acetonitrile as the mobile phase. Finally, sucralose content is calculated by an external standard method (based on peak area). - Sucralose moisture analysis
We use the quantitative reaction of iodine and sulfur dioxide with water to determine the moisture content of sucralose. Sucralose should not contain more than 2.0% moisture. - Incandescent residue of sucralose
We cauterize sucralose samples in a high-temperature environment. The residue of sucralose must not exceed 0.7%.
Publication Data
Technology: 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT)
Journal: Frontiers in Nutrition
IF: 5
Published: 2020
Results: In this article, the authors analyzed and assessed the effect of sucralose on mitochondrial bioenergetics produced in the intestinal epithelial cell line Caco-2 using the MTT technique. Low doses of sucralose stimulated mitochondrial respiration for 3 h and increased mitochondrial transmembrane potential and cytoplasmic Ca2+, as measured by oxygenography. In contrast to other cell types, the increase in mitochondrial respiration was insensitive to inhibition of mitochondrial Ca2+ uptake. These findings suggest that sucralose alters the dynamic balance of energy in enterocytes, thereby contributing to its effects on organismal metabolism.
Fig.1 Reductive effect of sucralose on cells. (Bórquez, et al., 2020)
Applications
- Professional sucralose analysis helps scientists delve deeper into the physiological functions of sucralose.
- A comprehensive analysis of sucralose can be used to assess its effects on the human body, providing an important basis for drug safety assessment.
- The analysis of sucralose helps scientists to explore the best drug formulations for optimal efficacy and stability.
Advantages
- Our analysis team is equipped with advanced Pharmaceutical and Biological Analysis techniques to perform sucralose analysis accurately.
- We have proven processes and experience in analysis method selection and sample handling to provide high-quality sucralose analysis services.
- We have strong data interpretation capabilities and provide our clients with scientific and reliable conclusions and recommendations based on our comprehensive interpretation of sucralose analysis results.
CD BioGlyco is a professional company specializing in biological research, with world-leading technology for the analysis of pharmaceutical excipients. Our professional team provides custom analysis protocols according to client needs and sample characteristics, ensuring that pharmaceutical excipient analysis results are tailored to the client's actual needs. Please feel free to contact us if you have any questions in the field of pharmaceutical excipient analysis. We will respond on time to meet your needs.
Reference
- Bórquez, J.C.; et al. Sucralose stimulates mitochondrial bioenergetics in Caco-2 cells. Frontiers in Nutrition. 2020, 7: 585484.
