Lactose plays an important role in carbohydrate metabolism. CD BioGlyco provides accurate, reliable, and reproducible lactose analytical services in milk, milk powder, functional beverages, health products, and pharmaceuticals.
Lactose, also known as β-D-galactopyranosyl-(1→4)-D-glucose, is a reducing disaccharide formed by the condensation of galactose and glucose, and both α-anomeric and β-anomeric are present. It is the major carbohydrate in most mammalian milk and makes an important contribution to the nutritional needs of newborns. Due to its physiological and functional properties, lactose has been widely used in the food and pharmaceutical industries. For example, lactose can be used as a free-flowing or agglomerating agent to enhance food flavors or as a diluent for colors, flavors, or enzymes. In the pharmaceutical industry, lactose is widely used as a drug carrier because it is safe, highly refined, and not sweet.
Fig.1 Lactose structure. (Wikipedia)
After lactose is internalized into cells, it is metabolized through Leloir and tagatose-6-phosphate pathways. In the Leloir pathway, lactose is hydrolyzed into glucose and β-galactose by β-galactosidase. β-Galactose is then transformed into glucose-1-phosphate via galactose mutarotase (GALM), galactose kinase (GALK), galactose-1-phosphate uridylyltransferase (GALT), and uridine diphosphate-galactose 4'-epimerase (GALE). In the tagatose-6-phosphate pathway, lactose-6-phosphate is hydrolyzed to glucose and galactose-6-phosphate by phospho-β-galactosidase (lacG). Galactose-6-phosphate is then transformed into glyceraldehyde-3-phosphate (GPDH) and dihydroxyacetone phosphate (DHAP) via galactose-6-phosphate isomerase (lacAB), tagatose-6-phosphate kinase (lacC) and tagatose-1,6-diphosphate aldolase (lacD).
Fig.2 Lactose metabolic pathway. (Iskandar, 2019)
As an important part of carbohydrate metabolism, lactose affects the body's energy supply, mineral absorption, immunity, and bones. Based on our comprehensive Carbohydrate Metabolism Analysis solutions, we provide lactose analysis services in milk, milk powder, functional beverages, health products, pharmaceuticals, and other samples. Not only can we determine the lactose content in various samples by high-performance liquid chromatography (HPLC). We also provide accurate, reliable, and reproducible lactose analysis by ion chromatography with pulsed amperometric detector (IC-PAD). In addition, we provide custom lactose analysis services based on your specific needs.
CD BioGlyco has been committed to carbohydrate metabolism research for many years and has accumulated rich experience. We provide accurate, reliable, and reproducible lactose analysis services for our global clients. If you are interested in our services, please feel free to contact us, we are looking forward to being your research assistant in the field of carbohydrate metabolism analysis.