Maltose is the hydrolysis product of starch and glycogen and is found mainly in malted grains. Based on our comprehensive carbohydrate metabolism analysis solutions, CD BioGlyco provides maltose analytical services for cooked sweet potatoes, wheat, fruit juice, nectar, and other samples.
Maltose is a disaccharide formed by the condensation of two units of glucose through α-(1,4) glycosidic linkage. It was first discovered by the French scientist Augustin-Pierre Dubrunfaut but was not widely accepted. It wasn't until 1872 that Irish chemist Cornelius O'Sullivan confirmed the existence of maltose. Maltose is an important biochemical substrate in the glucose chain, which can be prepared by the hydrolysis of starch and exists in foods such as potatoes and cereals. In the food industry, maltose is prepared into maltose syrup and is widely used in bread, beer, fruit juice, and other foods. In the pharmaceutical industry, maltose can be used as an intravenous solution to inhibit bacterial growth. And maltose syrup can be used as a medicine to promote gastrointestinal motility.
Fig.1 Maltose structure. (Wikipedia)
Like glucose, maltose is a reducing sugar that can be broken down by maltase to produce glucose. In E. coli, maltose enters the cytoplasm and is disproportionated to produce glucose and glycosylated receptor molecules. Glucose phosphorylation and phosphorolysis of glucosylated receptors produce hexose phosphates. In the human body, maltose is broken down by various maltase enzymes to produce glucose molecules. Glucose is broken down to provide energy for the body or stored as glycogen. In plants, maltose is abundantly present in malt as a product of β-amylase degradation of starch.
Fig.2 Proposed pathways for maltose metabolism in E. coli and Arabidopsis. (Ruzanski, 2013)
At CD BioGlyco, we have developed complete Carbohydrate Metabolism Analysis solutions, providing maltose analysis services for cooked sweet potatoes, wheat, fruit juice, nectar, and other samples. Our methods include:
HPLC is the most commonly used technique for determining the content of maltose, sucrose, glucose, etc. in samples. This method has shown the advantages of being precise, selective, and sensitive. We can determine maltose content by HPLC in cooked sweet potatoes, wheat, fruit juice, nectar, and other samples. Our detectors include refractive index detection (RID) and evaporative light scattering detector (ELSD).
HPAEC-PAD can direct determine carbohydrate content without derivatization and simple sample handling. We can determine maltose content in dietary supplements by HPAEC-PAD.
CD BioGlyco has been committed to carbohydrate metabolism research for many years and accumulated rich experience. We provide accurate and reproducible maltose analysis services for our clients. If you are interested in our services, please feel free to contact us, we are looking forward to being your research assistant in the field of carbohydrate metabolism analysis.