Your Trusted Partner for Custom In Vivo Glycobiology Disease Model with CRISPR

CD BioGlyco is a professional company providing Glycobiology Disease Model Development Services to clients such as Glycobiology Disease Model Construction, Custom In Vivo Glycobiology Disease Model, In Vivo Glycobiology Disease Model Screening, and In Vitro Glycobiology Disease Model Screening services. Our lab uses a specialized clustered regularly interspaced short palindromic repeat sequence (CRISPR) system to generate glycobiological disease models. Genome editing based on the CRISPR-CRISPR associated protein 9 (CRISPR/Cas9) system precisely introduces mutations in the target DNA sequence. This accelerates and advances the creation of customized animal models.

Generation of In Vivo Glycobiology Disease Model

Based on advanced CRISPR systems, we construct genome-editing animal models using microinjections, electroporation, and genome editing via oviductal nucleic acid delivery (GONAD). CD BioGlyco offers a wide range of gene editing services including point mutations (substitutions), small sequence insertions, knockin, large deletion, and chromosomal rearrangements (translocations, duplications, and inversions). We provide not only the common plasmid-based CRISPR/Cas9 but also CRISPR/Cas13, Cas9 mRNA/gRNA, and Cas9/gRNA ribonucleoproteins (RNPs). Based on the characteristics of the client's model, our researchers recommend the most suitable construction method for you. Our researchers achieve high-efficiency targeting by inducing double-strand breaks at the expected locus via CRISPR/Cas. Meanwhile, we provide in vivo enhancer characterization services.

CD BioGlyco mainly provides the following several services:

• CRISPR-Pro-based mouse & rat knockout service

CD BioGlyco provides the CRISPR/Cas9 system as a powerful gene editor for efficient and high-quality production of gene knockout mice and rats. Our researchers co-inject Cas9 mRNA and enhanced green fluorescent protein (EGFP) sgRNA into transgenic animals to achieve CRISPR/Cas9-mediated EGFP knockout. Common knockout genes include, but are not limited to ATP6V1H gene, Notch3 gene, Fah gene, Hpd gene, Asl genes, SOX9 gene, Irx3 gene, Irx5 genes, p53 gene, LKB1 gene, KRAS gene and so on. We offer double, triple, or multiple knockout services depending on client needs.

• CRISPR-Pro-based mouse & rat point mutation service

One or more nucleotides in the genome of a point mutant mouse and rat are replaced by a variant nucleotide. This may result in in-frame amino acid changes or code-shifting mutations in the protein sequence. With the support of CRISPR/Cas9, we use DNA double-strand break (DSB)-induced homologous recombination (HR)-dependent or independent repair mechanisms to generate point mutation mouse and rat. We obtain conditional mutant animals by co-injection of zygotes with Cas9 mRNA, different guide RNAs (sgRNAs), and DNA vectors of different sizes.

• CRISPR-Pro-based mouse & rat large-fragment knockin service

Based on homology-directed repair (HDR), our researchers induce precise large-fragment knockin service at the single nucleotide level. We inject HDR-targeted embryonic stem (ES) cells into wild-type host blastocysts to generate knockin mouse and rat strains. Our researcher achieves a one-step generation of knockin mouse and rat for targeted genes by injecting Cas9 mRNA in combination with sgRNA and single-stranded DNA oligonucleotide complexes/constructs into the cytoplasm of a zygote.

• CRISPR-Pro-based knockdown mice generation service

With the high-quality CRISPR/Cas9 system, we generate knockdown mice models by inducing insertion deletions that cause shifted codons. CRISPR-induced insertion deletions at the junction of exons and introns caused selective splicing and generated a wide variety of different mRNAs. Our researchers introduce these mRNAs into premature termination codons, leading to decreased expression of specific genes.

Publication

Frequently Asked Questions

• Why choose the CRISPR system to generate glycobiology disease models?
  • In CRISPR/Cas9, Our researchers need to clone only 19 nucleotides, i.e., nucleotides containing the appropriate targeting sequences, into a plasmid encoding both gRNA and Cas9. Other systems require the cloning of much larger sequences to encode the motif and repeat modules, respectively.
  • CRISPR/Cas9 demonstrates the highest genome editing efficiency in human and mouse cells.
• What are the advantages of CRISPR-pro?
  • CRISPR-pro is highly efficient and easily programmable making it possible to precisely edit the genome.
  • The sgRNA must be an exact match to the genomic sequence to be sheared. It has high-targeting accuracy.
  • CRISPR-pro can be applied directly to embryos to facilitate program development.

Advantages of Us

• The CRISPR/Cas13 we offer recognition of a single-stranded RNA, random degradation of target and non-target RNA, and no need to use a protospacer-adjacent motif (PAM) at the target site.
• Our lab provides a CRISPR system for the easy construction of sequence-specific nucleases and the efficient introduction of mutations.
• Depending on the needs of our clients, we provide comprehensive in vivo glycobiological disease model design, development, customization, and validation services.

CD BioGlyco attaches great importance to the cycle time and quality of our services and is committed to providing the most satisfactory solutions for our clients. Our operators all have rich backgrounds in gene editing. We are committed to promoting the development of our client projects. Please feel free to contact us.