Glucans have an important role in biomedical applications. Glucans can replace a portion of whole blood during blood transfusion as a plasma volume expander. Glucans can also activate macrophages and neutrophilic white blood cells, etc. It can increase the content of leukocytes, cytokinin, and special antibodies, and comprehensively stimulate the immune system. In addition, gels made of dextran are often used for biochemical separations, such as column chromatography. A chemically modified dextran obtained by fermentation of sucrose has a cross-linked mesh structure in its molecules, which can be used as a molecular sieve to separate low molecular weight compounds from high molecular weight compounds.
Fig.1 Structure of glucan. (CD BioGlyco)
CD BioGlyco offers analytical testing solutions for a wide range of Polysaccharides.
First of all, we offer the following services in testing the content of glucan:
Secondly, for the structure testing of glucan, we offer the following determination services:
Fig.2 Analytical procedure of glucan. (CD BioGlyco)
Paper Title: Structure analysis of β-glucan in barley and effects of wheat β-glucanase
Technology: Enzymatic determination of glucan
Journal: Journal of Cereal Science
Results: The structure of β-glucan as a whole was determined based on the distribution of oligosaccharides released from β-glucan in the three fractions after digitonin digestion. The β-glucan fragments were analyzed using principal component analysis (PCA). The variable DP3/DP4 mainly contributed to the first principal component, while fragments with DP ≥ 5 and sum DP5+DP6 were explained by the second principal component with DP5+DP6 being the most influential variable. In the scores plot a specific clustering was observed as the water extractable β-glucans of variety Karmose, SW 28708, and SLU 7 were found in the upper left quarter of the plot. β-Glucan quantification was performed on the water-insoluble fraction (WU) and on the fraction "WU-WhE-Ins" to highlight the proportion of β-glucan released in the supernatant by the wheat enzymes, given by the difference calculated between the amount of β-glucan in the two fractions The results showed that from the insoluble population of β-glucan 52.3% was released in variety SLU 7, 45.3% in Gustav and 44.1% in SW 28708.
CD BioGlyco has a professional research team and world-leading experimental capabilities with many years of experience in glucan research. We help our clients to solve the problems of glucan research as much as possible. If you are interested in our services, please feel free to contact us.