Glycan Microarray Assay

Perfect Glycan Microarray Analysis: Experience the Precision

Glycan microarrays are powerful tools for researching the functions and roles of glycans in organisms. Previously, CD BioGlyco has established a powerful Glycobiology Microarray Platform to provide clients with different kinds of comprehensive microarray analysis services. At present, CD BioGlyco has further constructed a Glycan Display Platform on top of this platform to provide clients with a full range of glycan microarray assay services.

Construction of glycan microarrays
CD BioGlyco provides glycan microarray constructs that include but are not limited to, the following.
- Covalently linked mono- (di-) glycan microarrays
  Glycan microarrays are formed by immobilizing mono- (di-) glycans by covalent linkage on a glass sheet carrier encapsulated with a monolayer of activating groups. We usually use fluorescent dyes to label and detect the binding specificity of the mono- (di-) glycan probes.
- Non-covalently adsorbed polysaccharide microarrays
  Polysaccharides or glycoproteins are immobilized by non-covalent adsorption on a surface hydrophobic carrier. The carriers we offer contain nitrocellulose membranes and polystyrene membranes.
- Oligosaccharide microarrays
  First, we immobilize long-chain aliphatic alkyne on the surface of solid-phase carriers by noncovalent adsorption. Second, the oligosaccharides are immobilized using cyclization-addition reactions to construct oligosaccharide microarrays.

Fig.1 Workflow of glycan microarray assays. (CD BioGlyco)

Based on the multiple glycan microarrays constructed, we provide detection services that include but are not limited to:

100 Glycan Microarray

CD BioGlyco helps researchers detect the binding partners for 100 diverse glycans in various biological samples such as serum, plasma, cell cultures, etc.

100 N-glycan Microarray

We provide 100 N-glycan microarray services to help researchers study the binding properties of different biological samples to an array of 100 basic N-glycans.

Sialic Acid Microarray

We provide sialic acid microarray service to help our customers discover glycan-binding proteins and viruses interacting with various modified sialic acids.

Microbial Glycan Microarray Assay

We offer an advanced microarray technology platform for the detection of specificity of host factors directly involved in host-pathogen interactions

Human Milk Oligosaccharide (HMO) Microarray Assay

The microarrays we have constructed include a variety of human lactide structures.

Sialylated Oligosaccharides Microarray Assay

We perform multi-parameter assays on multiple samples at the same time using sialylated oligosaccharides as the core of the constructed sialylated oligosaccharides microarray.

Glycosaminoglycan (GAG) Microarray Assay

We use GAG as a source to construct microarray arrays that efficiently screen for GAGs recognized by binding proteins.

ABH Blood Type I-VI Antigen Microarray Assay

This glycan microarray demonstrates the glycan determinant clusters of the human blood group structure and determines the presence of specific antibodies.

Synthetic Glycan Microarray Assay

We have established N- and O-glycan microarrays that are capable of analyzing multiple samples (e.g., proteins, cells, antibodies, cell lysates, and viruses) simultaneously.

Workflow

Sample processing and labeling

Glycan microarray assays are suitable for many types of samples (glycan-binding proteins, antibodies, cells, tissues, viruses, etc.). Depending on the type of sample, we perform initial processing, followed by fluorescent or other labeling of the biomolecules to be detected.

Microarray hybridization and washing

The labeled samples are incubated with the glycan microarrays to allow specific binding of the molecules to be detected to the glycans. The temperature, incubation time, and buffer conditions are strictly controlled to ensure optimal binding. At the end of hybridization, unbound samples and non-specifically bound material are removed.

Data analysis

A high-resolution microarray scanner is used to scan the chip after hybridization to obtain signal intensity data. Afterwards, we use professional bioinformatics software to process and analyze the raw data, including quantification, differential binding analysis, and cluster analysis.

Applications

Glycan microarrays can be used to rapidly analyze the glycan binding properties of proteins, including plant and animal lectins, antibodies, and growth factors.
Glycan microarrays can be used to probe the binding properties of pathogen glycans.
Glycan microarrays can be used to determine the affinity glycans of quantitatively bound proteins.

Advantages

We have a professional R&D team with in-depth research and rich experience in the construction and application of glycan microarray.
We provide custom glycan microarray assay services according to the client's requirements. Including the construction method of glycan microarray, the type of solid phase carrier, and so on.
Every solution we provide has gone through strict quality testing to ensure client satisfaction.

Publication Data

Technology: Glycan microarray

Journal: Molecular & Cellular Proteomics

IF: 6.1

Published: 2024

Results: This article introduces us to the types of glycan-binding proteins (GBPs), types of glycan microarrays, and the advantages of glycan microarrays in analyzing GBPs. Various glycan types can be placed on glycan microarrays, such as O-linked glycans, N-linked glycans, GPI-anchored glycans, and salivary acid-based glycans. Various glycan array methods can be constructed by strategies such as shotgun glycan microarrays. The constructed glycan microarrays are used to analyze lectin binding specificity, organism interactions, antibody-glycan binding, etc.

Fig.1 GBP and glycan binding analysis. Fig.1 Comparison of multiple GBP binding specificities. (Heimburg-Molinaro, et al., 2024)

Frequently Asked Questions

CD BioGlyco has several years of experience in analyzing glycan microarrays. Now, we have established an advanced glycan display platform, which provides every client with high-quality glycan microarray assay service. If you are interested in our services, please feel free to contact us.

Associated Services

(CD BioGlyco)

Reference

Heimburg-Molinaro, J.; et al. Insights into glycobiology and the protein-glycan interactome using glycan microarray technologies. Molecular & Cellular Proteomics. 2024: 100844. (Open Access)

This service is for Research Use Only, not intended for any clinical use.

Quick Links