Glycan Microarray Assay

Glycan microarray assay is a high-throughput screening tool frequently used in glycobiology. CD BioGlyco provides glycoprobe library and advanced detection approach to support customers' research.

Background

Compared with nucleic acids and proteins, carbohydrates are more diverse in structure, because it not only has more structural units, but each structural unit can form more than two connecting structures. Carbohydrates mediate many important biological processes, for example, pathogens use carbohydrates as receptors to attach to host cells and invade the cells; human life begins with the interaction between sialyl LewisX glycan on the egg and the egg-binding protein on the sperm. The diversity of carbohydrates and their biological significance has promoted the development of tools for high-throughput analysis of the interactions between carbohydrates and their potential binding partners, most importantly, glycan-binding proteins (GBPs). Many conventional methods for detecting the interaction between glycans and GBP, such as surface plasmon resonance (SPR), isothermal titration calorimetry (ITC) and enzyme-linked lectin assay (ELLA), can be time-consuming and require significant amounts of material.

As a result, researchers have invented glycan microarrays (also called carbohydrate microarrays or glycochips), which have become a powerful high-throughput tool for studying the interaction between carbohydrates and macromolecules (lectins, antibodies, toxins, and viruses). The glycan microarray allows the screening of hundreds of binding interactions in a single experiment using minimal material. In addition, the structure and appearance of the array surface can be changed, allowing many different combinations to be evaluated in one experiment.

Fig.1 Workflow of printed glycan microarrays with fluorescent readout. (Geissner, 2016)

Our Services

An important prerequisite for any glycan array experiment is a suitable glycan library that is subsequently surface-immobilized. Use one or more predicted interaction partners to detect on its surface and detect the GBP bound to each glycan separately. CD BioGlyco provides an appropriate glycan library according to needs and subsequently immobilizes it stably on the microwell plates. Experimental results can be obtained through incubation and high-precision detection methods.

Application

• Identification of biomarkers for cancer and infection
• Profiling of immune responses to vaccines
• Characterization and surveillance of influenza strains
• Glycan function research

Advantages of Us

• Advanced developing technology and data processing methods
• Highly stable glycoprobe
• Professional researchers
• Customized service

CD BioGlyco has quite a wealth of experimental experience in glycan microarray analysis. Our advanced experimental equipment and well-trained research personnel provide a guarantee for customers' experimental results. If you are interested in our services, please contact us for more detailed information.

Reference:

1. Geissner, A.; Seeberger, P.H. Glycan arrays: From basic biochemical research to bioanalytical and biomedical applications. Annu Rev Anal Chem. 2016, 91(1): 223-247.