Invertebrate Model-based In Vivo Screening Service
Unlocking the Power of Invertebrate Studies for Advanced Screening Solutions
Invertebrates, with their simple systems and short lifespans, allow easy genetic manipulation of their genomes and are ideal Models for Researching Glycobiology Diseases. At CD BioGlyco, we have established a specialized In Vivo Glycobiology Disease Model Screening platform covering a wide range of specific animal models. Here, we provide high-quality invertebrate model-based in vivo screening services to our clients.
- Constructing invertebrate models
Based on the characteristics of glycobiology diseases, we choose suitable invertebrates to serve as model organisms. For example, in the field of glyco-gene research, we choose Drosophila as a model to study the function of specific glyco-gene using genetic tools and glyco-gene mutation libraries. We use the silkworm model to research the characteristics of diseases due to protein glycosylation.
Moreover, we utilize gene editing technologies such as CRISPR-Cas9 to perform gene knockouts, knock-ins, or mutations to introduce specific gene mutations or markers in model animals to mimic the genetic background of human glycobiology diseases. - Screening for glycan biomarkers and glycan genes
We screen for possible glyco-biomarkers by researching phenotypic changes in model animals. We utilize electrochemical techniques, fluorescence microscopy, and molecular biology methods to research mechanisms of disease occurrence. Meanwhile, we provide polymerase chain reaction (PCR), Western blotting, mass spectrometry (MS), and other techniques to validate and analyze the screened glyco-biomarkers or genes.- Metabolomics analysis: analyzing metabolites in samples using high-throughput techniques such as MS or nuclear magnetic resonance (NMR) to look for glyco-biomarkers associated with phenotypic changes.
- Genetically encoded metabolic glyco-biomarkers: cell-specific glyco-biomarkers and analysis in living animals using genetically encoded metabolic glyco-biomarkers.
- Validating results
We utilize comparative experiments to validate the function and importance of the screened glyco-biomarkers or glyco-genes.
To enhance the reproducibility when replicating the animal model, we keep in mind every aspect of the experiment, such as the animal species, the experimental environment, the sequence of operation of the researchers, etc., to ensure the consistency of the conditions of the subsequent experiments.
Here, the specific invertebrate-based in vivo screening services we provide to our clients include but are not limited to, the following.
Drosophila Melanogaster Model-based In Vivo Screening Service
Drosophila Melanogaster's short developmental time, high genome-wide similarity to humans, and ability to express any gene from a vertebrate in its cells and tissues make it a powerful model for large-scale in vivo glyco-gene screening experiments.
Caenorhabditis Elegans Model-based In Vivo Screening Service
The use of CRISPR-Cas9 gene editing technology allows precise manipulation of genes at the individual nucleotide level in Caenorhabditis Elegans. Therefore, Caenorhabditis Elegans is a more idealized model for screening glyco-genes.
Silkworm Model-based In Vivo Screening Service
Blood glucose regulation in silkworms is similar to that of vertebrates, and therefore, it is possible to screen for genes that regulate and suppress elevated glucose levels in diabetic patients using the silkworm model.
Bee Model-based In Vivo Screening Service
In bees, ribonucleases (aRNAses) provide gentle cleavage of phosphodiester bonds in RNA. Bee is a new experimental system for screening anti-RNA virus compounds.
Publication Data
Technology: Silkworm model
Journal: Scientific Reports
IF: 4.6
Published: 2016
Results: In this paper, the authors constructed an in vivo evaluation system based on silkworms to screen for substances that cause elevated blood glucose levels in organisms. The authors found that glucose levels in silkworms were significantly suppressed after adding an α-glucosidase inhibitor to a sucrose-containing food. The authors also used a sucrose-induced hyperglycemic silkworm model to screen for a lactic acid bacteria capable of inhibiting lymphatic glucose levels in silkworms. These results suggest that postprandial hyperglycemic silkworms induced by a diet containing sucrose are a useful model for evaluating chemicals and lactobacilli that inhibit elevated blood glucose levels.
Fig.1 Scheme for the preparation of samples of silkworm. (Matsumoto, et al., 2016)
Applications
- The invertebrate model-based in vivo screening service can be used for high-throughput screening of drugs to find the most suitable drug combinations.
- The invertebrate model-based in vivo screening service can be used to provide new directions for clinical disease discovery and therapeutic target screening.
- The invertebrate model-based in vivo screening service can be used to functionally analyze human variants of unknown pathogenicity in an appropriate cellular or molecular context.
Advantages
- We have advanced biological techniques to provide custom animal models to our clients.
- We have a wide range of vertebrate or invertebrate animal models to choose from, ensuring that we provide our clients with efficient projects.
- We have an attentive 24h after-sales service.
CD BioGlyco is a professional glycobiology research company, providing professional and reliable glycobiology disease model development services to clients all over the world, Based on this, we have conducted thorough research on many types of animal models, covering both in vivo and in vitro glycobiology diseases screening. Please feel free to contact us if you are interested in our animal model development and disease screening services.
Reference
- Matsumoto, Y.; et al. An in vivo invertebrate evaluation system for identifying substances that suppress sucrose-induced postprandial hyperglycemia. Scientific Reports. 2016, 6: 26354.
