CD BioGlyco is constantly optimizing and expanding the applications of the NGL display array based on the Glycan Display Platform. Our NGL display array is used for high-throughput screening and characterization of carbohydrate-protein interactions. The process includes steps such as synthesis of artificial glycolipids, immobilization of NGLs, high-throughput screening, and protein binding analysis. To improve the detection range of the NGL display array and the accuracy of the results, we optimize each step of the experiment.
Fig.1 Process of NGL display array. (CD BioGlyco)
The NGL display array first involves the synthesis of artificial glycolipids, i.e., phosphatidylethanolamine type aminolipids conjugated to mixed glycans or pure glycans. We synthesize artificial glycolipids by enzymatically or chemically attaching specific glycans to lipid fractions. Arrays with different glycan motifs are generated by changing the structure of the glycan attached to the lipid. We screen appropriate lipids and glycans to ensure the reliability and reproducibility of results.
The NGL display array also involves the immobilization of artificial glycolipids onto solid supports such as slides, nitrocellulose membranes, and so on. We achieve the immobilization of artificial glycolipids by covalent attachment or non-covalent interactions. The method of attachment depends on the nature of the solid support and lipid anchor used.
The NGL display array screens multiple carbohydrate structures simultaneously against a set of proteins. We use high-throughput screening to identify specific protein-carbohydrate interactions, analyze binding preferences, and understand recognition patterns.
The array format facilitates qualitative and quantitative analysis of the binding of artificial glycolipids to proteins. We probe protein samples, such as lectins or antibodies, against arrays. We use a variety of methods to detect the binding interactions of artificial glycolipids with proteins, including fluorescence, radioactivity, or enzymatic labeling. Final results on the specificity, selectivity, and affinity of protein-carbohydrate interactions are obtained from the binding patterns.
Our NGL arrays are used by our clients in a variety of studies, including the study of processes such as cell adhesion and signaling, carbohydrate recognition events, and more. We develop detailed, customized protocols according to client requirements. We also use the NGL array in conjunction with other glycan microarrays to provide our clients with a more comprehensive interaction analysis.
Fig.2 Applications of NGL array. (CD BioGlyco)
Technology: NGL display array
Journal: Journal of Biological Chemistry
IF: 4.651
Published: 2012
Results: In this study, the carbohydrate-binding specificity of Toxoplasma gondii microparticle protein (TgMIC4) was analyzed based on NGL display arrays. These microarrays consisted of a set of 400 lipid-linked oligosaccharide probes representing sequences derived from bacterial and fungal polysaccharides as well as different mammalian glycan sequences and their analogs. The results show that TgMIC4-A5 and TgMIC4-A56 exhibit good binding to a variety of oligosaccharide probes terminating in β-galactose with similar binding profiles.
Fig.3 Carbohydrate microarray analyses of TgMIC4-A5. (Marchant, et al., 2012)
CD BioGlyco provides a very reliable NGL display array analysis service to clients all over the world. Welcome to contact us to learn more about artificial glycolipid synthesis, NGL immobilization, NGL display array application, and so on. Our professional researchers will surely give you a very satisfactory service experience.
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