Group A Meningococcal Polysaccharide Vaccine Evaluation Service
Efficacy and Accuracy for Group A Meningococcal Polysaccharide Vaccine
Whether you are looking for Pharmaceutical Analysis, Biological Evaluation, or Pharmaceutical Excipient Analysis, CD BioGlyco has the best-in-class analytical technology and professional operators to meet all the needs of our clients in Pharmaceutical and Biological Analysis. Meningococcal polysaccharide vaccine is a classical vaccine containing the podocarboxylic polysaccharide antigen. It is commonly used to prevent epidemic meningitis caused by Neisseria meningitides. Our team provides high-quality evaluation for group A and C meningococcal polysaccharide vaccine, group A and group C meningococcal conjugate vaccine, and group ACYW135 meningococcal polysaccharide vaccine.
- Bulk testing
- Identification
CD BioGlyco observes the precipitates formed by the samples to be tested with antibodies against group A Neisseria meningitidis by double immunodiffusion assay. - Basic testing
- Total solid
- O-Acetyl content
- Phenol residue
- Protein content
- Phosphate content
- Sterility test
- Nucleic acid content
- Polysaccharide molecular size distribution
- Bacterial endotoxin test
- Identification
- Final bulk testing
- Sterility testing of semi-finished vaccines is very essential and we provide reliable sterility testing services to clients.
- Final product testing
- CD BioGlyco provides accurate and high-quality basic testing including load discrepancy tests, moisture determination, polysaccharide content determination, polysaccharide molecular size distribution determination, and abnormal toxicity tests.
- CD BioGlyco provides osmotic concentration and pyrogen testing services. Our researchers use measurements of the freezing point drop of a solution to indirectly determine its osmotic concentration. For the pyrogen testing, we inject a certain dose of vaccine, intravenously, into rabbits, and observe the rabbits' body temperature rise over a specified period to determine the limit of pyrogens contained in the vaccine.
- Diluent determination
- CD BioGlyco provides high-quality vaccine diluent assay services including visible foreign body inspection, pH measurement, sterility test, and bacterial endotoxin assay services.
Publication Data
Technology: Reverse transcription quantitative PCR (RT-qPCR), High-throughput transcriptome sequencing, Enzyme linked immunosorbent assay (ELISA)
Journal: Vaccines
Published: 2023
IF: 4.961
Results: This study provided the first comprehensive characterization of the lncRNA and mRNA profiles of the spleens of group ACYW135 meningococcal polysaccharide vaccine (MPV-ACYW135) and control mice, and the results showed that differentially expressed (DE) lncRNAs are associated with immune regulation in the mouse spleen. The vaccinated and control groups contained 405 DE lncRNAs and 52 DEmrna. The results by enrichment analysis showed that MPV-ACYW135 podoplanar polysaccharide antigen has the ability to activate T-cell-associated immune responses in the inoculation. Notably after inoculation, the lncRNA MSTRG.17645 may have a significant regulatory effect on its potential target gene Bach2.
Fig.1 The verification result by RT-qPCR. (Zhu, et al., 2023)
Applications
- Our evaluation service further facilitates polysaccharide vaccine development and characterization.
- Assessed group A meningococcal polysaccharide vaccines provide better prevention and control of infectious diseases such as influenza and pneumonia.
- Detection of immune response to group A meningococcal polysaccharide vaccine facilitates the study of transcriptomes of vaccine candidate genes.
Advantages of Us
- Our specialized analysts offer not only final vaccine assays but also bulk and final bulk assays.
- Our researchers strictly adhere to sterility conditions. The environment is tested before the inspection to ensure accurate data.
- For all animal-related tests, we offer a one-stop evaluation service from animal culture, injection planning, long-term observation to data reporting.
Based on advanced analytical systems, CD BioGlyco provides high-quality pharmaceutical and biological analysis services. Our efficient and highly satisfying services have been recognized by our clients all over the world. Don't hesitate to contact us whenever you need assistance.
Reference
- Zhu, N.; et al. Comparative transcriptome profiling of mRNA and IncRNA of mouse spleens inoculated with the group ACYW135 meningococcal polysaccharide vaccine. Vaccines. 2023, 11(8): 1295.
