Microbial glycan microarray assay is a high-throughput screening frequently used in glycobiology. CD BioGlyco provides advanced microarray technology platform and customizable services, and we have confidence to be your essential research assistant in the field of glycobiology.

Background

The interaction between glycan binding protein and their carbohydrate ligand is the basis of all life. Over the past decade or so, printed glycan arrays have helped elucidate the role of glycans in many biological processes, especially those of mammalian origin. Genomics methods also provide unprecedented insights into microbiology. However, the immune interaction between the hosts and various microbial groups remains elusive and difficult to study. Microbial microarray is the new method that can be used to explore this interaction. One of the main advantages of the microarray format is the inherent miniaturization of the method, which allows for sensitive and high-throughput analysis with very few samples. On the surface of a single microorganism, glycosyl determinants have been considered a key structure for host immune factor recognition. They usually represent unique antigenic determinants used to define specific strains. Therefore, the detection of host factors of various microbial glycans not only can identify interactions with individual microorganisms, but also provide key insights into the structure of these interactions.

Fig.1 Lectins interact with microbial glycans with high affinity. (Wu, et al., 2021)

Our Services

Microbial glycan microarray detection is a high-throughput, highly sensitive technology used to study the interactions between microorganisms (bacteria, fungi, viruses, etc.) and host glycans. By spotting glycan molecules from diverse microbial sources onto microarray chips to form a unique glycan library, and then incubating them with labeled target biomolecules, this method can reveal the key roles of microbial glycans in biological processes such as infection and immunity with high sensitivity and specificity.

Our microbial glycan microarray assay is similar to other types of microarrays. Isolate high-purity and biologically active bacterial glycans from different microorganisms or synthesize microbial glycans directly, then print them in an immobile phase and examine the printing efficiency. Incubate one or more predicted interaction partners that may have recognition activity with the microarray on its surface, and detect them individually by advanced detection equipment. Our microbial glycan microarray platform can be used for rapid and high-throughput screening. Customized arrays can also be printed to meet project needs. Our experts can provide a detailed analysis of the results upon request.

Workflow

• Microbial glycan microarray construction: We select suitable microarray chip substrates and choose appropriate methods to immobilize microbial glycans onto the chip surface based on the structural characteristics of the glycans and the chip type.
• Sample preparation and labeling: We further process the samples according to their type, including additional purification to meet experimental requirements and labeling of target biomolecules.
• Microarray incubation and washing: Following an optimized experimental protocol, labeled biomolecular samples are incubated with the constructed microbial glycan microarray under precisely controlled conditions. A multi-step, gentle washing strategy is employed to efficiently remove unbound non-specific background signals.
• Detection and data processing: High-resolution images are obtained using a high-sensitivity microarray scanner. The fluorescence intensity at each glycan site is analyzed to identify glycans that specifically bind to the target biomolecules, and the binding results are subjected to in-depth biological interpretation.

Applications

• Host defense mechanism research: Microbial glycan microarray detection is used to gain a deeper understanding of how hosts recognize and respond to microorganisms, including exploration of pattern recognition receptors and immune cell activation.
• Identifying binding partners or assessing the specificity of newly developed probes: Microbial glycan microarray detection is used to discover molecules (binding partners) that interact with specific microbial glycans, or to precisely assess the binding affinity and specificity of newly synthesized/isolated biological probes, drug molecules, antibodies, or lectins toward different glycans.
• Microbial characterization and monitoring: Microbial glycan microarray assays are used to analyze the unique glycan expression profiles of different microbial strains or species, enabling precise strain identification, typing, and monitoring of pathogen epidemiological trends.

Advantages

• Through optimized chip design and detection methods, we achieve highly sensitive binding detection capable of capturing weak interactions.
• Microbial glycan microarrays accurately identify molecules that bind to target glycans, reducing false positives, improving data reliability, and aiding in a deeper understanding of complex molecular recognition mechanisms.
• Microbial glycan microarrays can be used to detect the binding of various types of biomolecules to glycans, including antibodies, lectins, receptor proteins, and more.

Publication Data

Frequently Asked Questions

CD BioGlyco has quite a wealth of experimental experience in microbial glycan microarray analysis. Our advanced experimental equipment and well-trained research personnel provide guarantee for customers' experimental results.

Customers can contact our employees directly and we will respond promptly. If you are interested in our services, please contact us for more detailed information.

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