CD BioGlyco has abundant experience in lipidomics analysis services. We expect to become customers' research assistants in ester phospholipid analysis.
The structure of ester phospholipids consists of a glycerol backbone linked to a phosphorylated “head” group including simply phosphate, phosphorylcholine, phosphorylethanolamine, phosphorylinositol, or phosphorylserine. Ester phospholipids further contain up to two ester-linked fatty acids.
The head group of ester phospholipids greatly impacts biological function. Phosphatidylcholine is the major phospholipid on the surface monolayer of all lipoproteins. Phosphatidylethanolamine (PE) is a multifunctional phospholipid required for mammalian development that is essential for a variety of cellular processes. It is a nonbilayer forming phospholipid containing a small polar head group diameter in proportion to its fatty-acid chains. Phosphatidylglycerol is a major lipid in many bacterial membranes. Phosphatidylserine is a phospholipid and is a component of the cell membrane. It plays a key role in cell cycle signaling, specifically in apoptosis. In conclusion, ester phospholipids are the major structural component of all biological membranes, from plasma membranes to organellar membranes. They are essential to regulating cellular functions directly and indirectly.
Fig.1 The structure of phosphatidylcholine. (Wikipedia)
ESI/MS of phospholipids represents one of the most sensitive, discriminating, and direct methods to assess alterations in the phospholipid content in biological tissues. CD BioGlyco provides an internally standardized, negative mode mass spectrometry approach for quantitative ester phospholipid analysis and structural determinations by electrospray ionization mass spectrometry (ESI/MS).
The advantages of ESI/MS for lipid analysis include: (1) the signal-to-noise ratio is two orders of magnitude higher than that found in other mass spectrometric approaches; (2) the relative intensity of molecular ions is linear to the mass of individual phospholipids over a 10000-fold dynamic range examined; (3) the ion intensity is rarely dependent on the nature of the phospholipid subclass or the individual molecular species; (4) the complete analysis of phospholipid classes, subclasses, and individual molecular species can be achieved without the need for prior chromatographic separation or derivatization, and (5) the repeatability of measurements from an identical sample is excellent (<5% of experimental error).
CD BioGlyco aspires to become the trusted solution provider of your first choice. Our advanced technologies and highly experienced staffs are committed to advancing your program. Our professional research teams provide customers with high-quality phospholipids analysis services. For further details, please don't hesitate to contact us.